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Serial Synchrotron X-Ray Crystallography (SSX)

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Protein Crystallography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1607))

Abstract

Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set. Measuring, processing, and merging many partial data sets of this kind requires new techniques which have since been implemented at several third-generation synchrotron facilities, and are described here. Among these, we particularly focus on the possibility of in situ measurements combined with in meso crystal preparations and data analysis with the XDS package and auxiliary programs.

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Acknowledgments

We thank Greta Assmann, Wolfgang Brehm, Martin Caffrey, Chia-Ying Huang, Vincent Olieric, Ezequiel Panepucci, Rangana Warshamanage, and all other members of the groups at the Swiss Light Source (Paul-Scherrer-Institute, Villigen, Switzerland), Trinity College (Dublin, Ireland) and University of Konstanz (Konstanz, Germany) for discussions and their contributions toward developing the methodology. We also thank Aaron Finke and Martin Caffrey for proofreading the manuscript and Rangana Warshamanage and Chia-Ying Huang for preparing the figures.

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Authors and Affiliations

  1. Department of Biology, Universität Konstanz, Box 647, D-78457, Konstanz, Germany

    Kay Diederichs

  2. Swiss Light Source, Paul Scherrer Institute, CH-5232, Villigen, Switzerland

    Meitian Wang

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  1. Kay Diederichs
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  2. Meitian Wang
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Correspondence to Kay Diederichs .

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Editors and Affiliations

  1. Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, USA

    Alexander Wlodawer

  2. Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, Illinois, USA

    Zbigniew Dauter

  3. Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland

    Mariusz Jaskolski

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Diederichs, K., Wang, M. (2017). Serial Synchrotron X-Ray Crystallography (SSX). In: Wlodawer, A., Dauter, Z., Jaskolski, M. (eds) Protein Crystallography. Methods in Molecular Biology, vol 1607. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7000-1_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7000-1_10

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