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KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress

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Peroxisomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1595))

Abstract

Many biological processes and cell fate decisions are modulated by changes in redox environment. To gain insight into how subcellular compartmentalization of reactive oxygen species (ROS) formation contributes to (site-specific) redox signaling and oxidative stress responses, it is critical to have access to tools that allow tight spatial and temporal control of ROS production. Over the past decade, the use of genetically encoded photosensitizers has attracted growing interest of researchers because these proteins can be easily targeted to various subcellular compartments and allow for controlled release of ROS when excited by light. This chapter provides guidance and practical advice on the use of po-KR, a peroxisomal variant of the phototoxic red fluorescent protein KillerRed, to address fundamental questions about how mammalian cells cope with peroxisome-derived oxidative stress.

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Acknowledgments

The authors are grateful to Ms. C. Lismont (KU Leuven, Belgium) for critical reading of the manuscript. This work was supported by grants from the KU Leuven (OT/14/100) and the Fonds voor Wetenschappelijk Onderzoek-Vlaanderen (Onderzoeksproject G095315N).

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Authors and Affiliations

  1. Laboratory of Lipid Biochemistry and Protein Interactions, Department of Cellular and Molecular Medicine, KU Leuven – University of Leuven, Leuven, Belgium

    Marc Fransen & Chantal Brees

Authors
  1. Marc Fransen
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  2. Chantal Brees
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Corresponding author

Correspondence to Marc Fransen .

Editor information

Editors and Affiliations

  1. College of Life and Environmental Sciences, Biosciences, University of Exeter, Exeter, United Kingdom

    Michael Schrader

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Fransen, M., Brees, C. (2017). KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress. In: Schrader, M. (eds) Peroxisomes. Methods in Molecular Biology, vol 1595. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6937-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6937-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6935-7

  • Online ISBN: 978-1-4939-6937-1

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