Abstract
A novel mini-electrochemical system has been developed for evaluating cytotoxicity of anticancer drugs based on trace cell samples. The mini-electrochemical system was integrated by using pencil graphite modified with threonine as working electrode, an Ag/AgCl reference electrode and a micropipet tip as electrochemical cell. The mini-electrochemical system dramatically reduces sample volumes from 500 μL in a traditional electrochemical system to 10 μL, and exhibits excellent electrocatalytic activity toward oxidation of purine from MCF-7 cells due to increased sensitivity provided by threonine. Moreover, the relationship between peak current and the cell concentration in the range from 3.0 × l03 to 7.0 × l06 cells/mL was studied, and a nonlinear exponential relationship between them was established over a wide concentration range. In evaluating the effect of anticancer drugs on cell viability, the results of drug cytotoxicity test based on cyclophosphamide were in close agreement with classical 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays. The proposed device is so simple, cheap, and easy to operate that it could be applied to single-use applications. The mini-electrochemical system proved to be a useful tool and can be applied to electrochemical studies of cancer cells as well as other biological samples such as proteins and DNA.
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Acknowledgments
We gratefully acknowledge the financial support of the funds from NSFC in China (51178092 and 81273132), the Nature Science Foundation of Heilongjiang Province (B2015013), a fund from Heilongjiang Province Department of Education (12531673), and a fund from Jiamusi University (Ljz2012-25).
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Wu, DM. et al. (2017). A Mini-Electrochemical System with Integrated Micropipet Tip and Pencil Graphite Electrode for Measuring Cytotoxicity. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_11
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DOI: https://doi.org/10.1007/978-1-4939-6911-1_11
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Publisher Name: Humana Press, New York, NY
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