Abstract
In this chapter, we describe the preparation of glucose-responsive vesicles (GRVs) and the fabrication of GRV-loaded microneedle-array patches for insulin delivery. The GRVs were formed of hypoxia-sensitive hyaluronic acid (HS-HA), the synthesis of which is presented in detail. We also describe the procedure to evaluate the in vivo efficacy of this smart patch in a mouse model of chemically induced type 1 diabetes through transcutaneous administration.
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Acknowledgment
This work was supported by the grants from the American Diabetes Association (ADA) to Z.G. (1-14-JF-29 and 1-15-ACE-21) and a grant from NC TraCS, NIH’s Clinical and Translational Science Awards (CTSA, NIH grant 1UL1TR001111) at UNC-CH.
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Yu, J., Zhang, Y., Gu, Z. (2017). Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles. In: Petrosko, S., Day, E. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 1570. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6840-4_17
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DOI: https://doi.org/10.1007/978-1-4939-6840-4_17
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