Abstract
The Extracellular Vesicle (EV) Array is based on the technology of protein microarray and provides the opportunity to detect and phenotype small EVs from unpurified starting material in a high-throughput manner (Jørgensen et al., J Extracell vesicles 2:1–9, 2013). The technology was established to perform multiplexed phenotyping of EVs in an open platform. This protocol outlines the microarray printing procedure followed by the steps of capture and detection of small extracellular vesicles from plasma/serum or cell culture supernatants. The principles of data treatment and analysis are thoroughly described as well.
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Abbreviations
- dAF488:
-
Dextran coupled with Alexa Fluor 488
- sEV:
-
small Extracellular vesicle
- IgG:
-
Immunoglobin gamma
- ON:
-
Overnight
- PBS:
-
Phosphate-buffered saline
- RT:
-
Room temperature
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Acknowledgments
The authors would like to thank M.D. Kim Varming and Dr. Evo K.L. Søndergaard for helping establish this protocol.
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Bæk, R., Jørgensen, M.M. (2017). Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array). In: Hill, A. (eds) Exosomes and Microvesicles. Methods in Molecular Biology, vol 1545. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6728-5_8
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DOI: https://doi.org/10.1007/978-1-4939-6728-5_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6726-1
Online ISBN: 978-1-4939-6728-5
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