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Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques

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Cardiac Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1521))

Abstract

Percutaneous antegrade coronary injection is among the least invasive cardiac selective gene delivery methods. However, transduction efficiency is quite low with a simple bolus antegrade injection. In order to improve the transduction efficiency using antegrade delivery, several additional approaches have been proposed.

In this chapter, we briefly discuss important elements associated with intracoronary delivery methods and present protocols for three different catheter-based antegrade delivery techniques in a preclinical large animal model. Despite the lower transduction efficacy relative to more invasive delivery techniques, antegrade techniques have the advantage of being clinically well established and having safer profiles which is important when treating patients with cardiac disease.

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Acknowledgments

This work is supported by NIH P50 HL112324, R01 HL119046, R01 HL117505, R01 HL128099, R01 HL129814, R01HL131404, & T32 HL007824 (R. J. H.), and a Transatlantic Leducq Foundation grant. We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health for providing some of the gene vectors used in these studies.

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Authors and Affiliations

  1. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA

    Shin Watanabe, Lauren Leonardson, Roger J. Hajjar & Kiyotake Ishikawa M.D.

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  1. Shin Watanabe
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  2. Lauren Leonardson
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  3. Roger J. Hajjar
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  4. Kiyotake Ishikawa M.D.
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Corresponding author

Correspondence to Kiyotake Ishikawa M.D. .

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Editors and Affiliations

  1. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York, USA

    Kiyotake Ishikawa

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Watanabe, S., Leonardson, L., Hajjar, R.J., Ishikawa, K. (2017). Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 1521. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6588-5_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6588-5_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6586-1

  • Online ISBN: 978-1-4939-6588-5

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