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Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors

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Drug Target miRNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1517))

Abstract

MicroRNAs (miRNAs) are involved in most cellular processes and are deregulated in several diseases. Antisense miRNA oligonucleotides (AMOs) therefore present novel therapeutic opportunities. Currently, in vivo delivery of AMOs often relies on high doses of nucleic acids, with nonspecific uptake by most tissues. Critically, AMOs accumulate in phagocytic cells where they can interfere with immune functions, such as the activation of Toll-Like Receptors (TLRs). In this chapter, we describe a method to assess the possible off-target effects of AMOs on TLR7, 8, and 9 sensing.

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Acknowledgments

The authors thank Frances Cribbin for her help with the redaction of this chapter. The authors are supported by funding from the Australian NHMRC (1062683 and 1081167 to M.P.G.); the Australian Research Council (140100594 Future Fellowship to M.P.G.); and the Victorian Government’s Operational Infrastructure Support Program.

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    Authors and Affiliations

    1. Centre for Cancer Research, Hudson Institute of Medical Research, 27-31 Wright St., Clayton, VIC, 3168, Australia

      Geneviève Pépin, Jonathan Ferrand & Michael P. Gantier

    2. Department of Molecular and Translational Science, Monash University, Clayton, VIC, 3168, Australia

      Geneviève Pépin, Jonathan Ferrand & Michael P. Gantier

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    1. Geneviève Pépin
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    2. Jonathan Ferrand
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    3. Michael P. Gantier
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    Correspondence to Michael P. Gantier .

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    1. BEROCEUTICA GmbH, Potsdam, Germany

      Marco F. Schmidt

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    Pépin, G., Ferrand, J., Gantier, M.P. (2017). Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors. In: Schmidt, M. (eds) Drug Target miRNA. Methods in Molecular Biology, vol 1517. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6563-2_9

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    • DOI: https://doi.org/10.1007/978-1-4939-6563-2_9

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-6561-8

    • Online ISBN: 978-1-4939-6563-2

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