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Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation

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Cell Microencapsulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1479))

Abstract

Microencapsulation of islets is a procedure used to immunoisolate islets in order to obviate the need for immunosuppression of islet transplant recipients. Although microencapsulated islets have routinely been transplanted in the peritoneal cavity, the ideal site for their engraftment remains to be determined. The omentum, a highly vascularized tissue, has been proposed as an alternative site for microencapsulated islet transplantation. An added benefit to the omentum is that implanted microcapsules can be easily retrieved for post-transplant evaluation. This chapter describes a collagenase-based procedure for the retrieval of microencapsulated islets following the harvest of omentum pouch site of transplantation.

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Acknowledgements

The authors would like to acknowledge financial support from the National Institutes of Health (RO1 DK080897) and the Vila Rosenfeld Estate, Greenville NC for the work in Dr. Opara’s laboratory at the Wake Forest Institute for Regenerative Medicine.

Also, research reported in this publication was supported by the National Institute of Biomedical Imaging and Bioengineering (NIBIB) of the National Institutes of Health under award # T32EB014836. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, 27157, USA

    John Patrick McQuilling, Rajesh Pareta, Samuel Pendergraft & Alan C. Farney

  2. Virginia Tech, Wake Forest University School of Biomedical Engineering and Sciences, Winston-Salem, NC, 27157, USA

    John Patrick McQuilling

  3. Department of Surgery, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA

    Clancy J. Clark & Alan C. Farney

  4. Wake Forest Institute forRegenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, 27157, USA

    Sivanandane Sittadjody

  5. Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA

    Emmanuel C. Opara

  6. Virginia Tech-Wake Forest School of Biomedical Engineering & Sciences (SBES), Wake Forest School of Medicine, Winston-Salem, NC, USA

    Emmanuel C. Opara

Authors
  1. John Patrick McQuilling
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  2. Sivanandane Sittadjody
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  3. Rajesh Pareta
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  4. Samuel Pendergraft
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  5. Clancy J. Clark
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  6. Alan C. Farney
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  7. Emmanuel C. Opara
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Corresponding author

Correspondence to Emmanuel C. Opara .

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Editors and Affiliations

  1. Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine, Winston-Salem, North Carolina, USA

    Emmanuel C. Opara

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McQuilling, J.P. et al. (2017). Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6364-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6364-5_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6362-1

  • Online ISBN: 978-1-4939-6364-5

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