Abstract
mRNA regulation by poly(A) tail length variations plays an important role in many developmental processes. Recent advances have shown that, in particular, deadenylation (the shortening of mRNA poly(A) tails) is essential for germ-line stem cell biology in the Drosophila ovary. Therefore, a rapid and accurate method to analyze poly(A) tail lengths of specific mRNAs in this tissue is valuable. Several methods of poly(A) test (PAT) assays have been reported to measure mRNA poly(A) tail lengths in vivo. Here, we describe two of these methods (PAT and ePAT) that we have adapted for Drosophila ovarian germ cells and germ-line stem cells.
Aymeric Chartier and Willy Joly contributed equally.
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Acknowledgements
Work in the Simonelig lab is supported by the CNRS UPR1142, ANR (ANR-2010-BLAN-1201 01 and ANR-15-CE12-0019-01), FRM (Equipe FRM 2013 DEQ20130326534), and AFM-Telethon (No 17110).
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Chartier, A., Joly, W., Simonelig, M. (2017). Measurement of mRNA Poly(A) Tail Lengths in Drosophila Female Germ Cells and Germ-Line Stem Cells. In: Buszczak, M. (eds) Germline Stem Cells. Methods in Molecular Biology, vol 1463. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4017-2_7
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DOI: https://doi.org/10.1007/978-1-4939-4017-2_7
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