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Characterization of Chemical and Physical Modifications of Human Serum Albumin by Capillary Zone Electrophoresis

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Capillary Electrophoresis of Proteins and Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1466))

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Abstract

Therapeutic proteins can easily undergo chemical or physical changes during their manufacturing, purification, and storage. These modifications might change or reduce their biological activity. Therefore, it is important to have analytical methodologies that are able to reliably detect, characterize, and quantify degradation products in formulations. Capillary Zone Electrophoresis (CZE) is very well suited for the analysis of proteins due to its relatively easiness of implementation, separation efficiency, and resolving power. We describe here a CZE method that allows separating more than nine forms in therapeutic albumin, including oxidized, glycated, and truncated forms. This method uses a polyethylene oxide (PEO) coating and a buffer composed of HEPES and SDS at physiological pH. The method is reproducible (RSD < 0.5 and 4 % for migration times and peak areas, respectively) and allows quantitation of albumin forms in pharmaceutical preparations.

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Acknowledgments

This work was supported by LFB Biotechnologies.

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Authors and Affiliations

  1. Institut Galien Paris-Sud, UMR 8612, Proteins and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, 5 rue J-B Clément, Châtenay-Malabry, 92290, France

    Anne-Lise Marie

  2. Institut Galien Paris-Sud, UMR 8612, Proteins and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Nguyet Thuy Tran & Myriam Taverna

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  1. Anne-Lise Marie
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  2. Nguyet Thuy Tran
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  3. Myriam Taverna
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Correspondence to Myriam Taverna .

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Editors and Affiliations

  1. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Nguyet Thuy Tran

  2. Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

    Myriam Taverna

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Marie, AL., Tran, N.T., Taverna, M. (2016). Characterization of Chemical and Physical Modifications of Human Serum Albumin by Capillary Zone Electrophoresis. In: Tran, N., Taverna, M. (eds) Capillary Electrophoresis of Proteins and Peptides. Methods in Molecular Biology, vol 1466. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4014-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-4014-1_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-4012-7

  • Online ISBN: 978-1-4939-4014-1

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