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In Vivo 19F-Magnetic Resonance Imaging of Adoptively Transferred NK Cells

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Natural Killer Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1441))

Abstract

In order to assess the biodistribution, homing, and persistence of adoptively transferred natural killer (NK) cell immunotherapies, there is a need for imaging methodology suitable for use in preclinical studies with relevance to clinical translation. Amongst the available approaches, 19F-MRI is very appealing for in vivo imaging due to the absence of background signal, enabling clear detection of 19F labeled cells in vivo. Here we describe a methodology for in vivo imaging of adoptively transferred NK cells labeled with 19F nano-emulsion, using clinically translatable technology of 19F/1H magnetic resonance imaging.

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Acknowledgments

This work was supported in part by funding from the Addis Faith Foundation to VG, CURE Childhood Cancer funding to DAL, and by MD Anderson Cancer Center’s Core Grant (P30-CA016672).

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Authors and Affiliations

  1. Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, 77054, USA

    Srinivas S. Somanchi, Bridget A. Kennis, Vidya Gopalakrishnan & Dean A. Lee

  2. Department of Imaging Physics, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX, 77054, USA

    James A. Bankson Ph.D.

Authors
  1. Srinivas S. Somanchi
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  2. Bridget A. Kennis
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  3. Vidya Gopalakrishnan
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  4. Dean A. Lee
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  5. James A. Bankson Ph.D.
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Corresponding author

Correspondence to James A. Bankson Ph.D. .

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Editors and Affiliations

  1. Division of Pediatrics, M.D. Anderson Cancer Center, Houston, Texas, USA

    Srinivas S. Somanchi

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Somanchi, S.S., Kennis, B.A., Gopalakrishnan, V., Lee, D.A., Bankson, J.A. (2016). In Vivo 19F-Magnetic Resonance Imaging of Adoptively Transferred NK Cells. In: Somanchi, S. (eds) Natural Killer Cells. Methods in Molecular Biology, vol 1441. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3684-7_27

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  • DOI: https://doi.org/10.1007/978-1-4939-3684-7_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3682-3

  • Online ISBN: 978-1-4939-3684-7

  • eBook Packages: Springer Protocols

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