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Application of Fluorescence Polarization in HTS Assays

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High Throughput Screening

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1439))

Abstract

Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

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Acknowledgments

The authors would like to thank Ray Unwalla of Wyeth Research for molecular modeling of E-76 and the E-76 based probes, and would like to thank Rebecca Shirk and Belew Mekonnen of Wyeth Research for collaboration on the TF/FVIIa, and would like to thank Shannon Stahler, Nina Kadakia, Gary Kalgaonkar, William Martin, Mariya Gazumyan, Pedro Sobers, and Jim LaRocque of Wyeth Research for contribution to the NR project, and would like to thank Richard Harrison of Wyeth Research for critical review of the chapter.

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Authors and Affiliations

  1. Immunology, Inflammation and Infectious Diseases Discovery and Translational Area Roche Pharma Research & Early Development, Roche Innovation Center Shanghai, Roche R&D Center (China) Ltd, 720 Cai Lun Road, Bldg 5, Pudong, Shanghai, 201203, China

    Xinyi Huang & Ann Aulabaugh

Authors
  1. Xinyi Huang
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  2. Ann Aulabaugh
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Corresponding author

Correspondence to Xinyi Huang .

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Editors and Affiliations

  1. Epizyme, Inc., Cambridge, Massachusetts, USA

    William P. Janzen

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Huang, X., Aulabaugh, A. (2016). Application of Fluorescence Polarization in HTS Assays. In: Janzen, W. (eds) High Throughput Screening. Methods in Molecular Biology, vol 1439. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3673-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3673-1_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3671-7

  • Online ISBN: 978-1-4939-3673-1

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