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Imaging Mitosis in the Moss Physcomitrella patens

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The Mitotic Spindle

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1413))

Abstract

At first glance, mitosis in plants looks quite different from that in animals. In fact, terrestrial plants have lost the centrosome during evolution, and the mitotic spindle is assembled independently of a strong microtubule organizing center. The phragmoplast is a plant-specific mitotic apparatus formed after anaphase, which expands centrifugally towards the cell cortex. However, the extent to which plant mitosis differs from that of animals at the level of the protein repertoire is uncertain, largely because of the difficulty in the identification and in vivo characterization of mitotic genes of plants. Here, we discuss protocols for mitosis imaging that can be combined with endogenous green fluorescent protein (GFP) tagging or conditional RNA interference (RNAi) in the moss Physcomitrella patens, which is an emergent model plant for cell and developmental biology. This system has potential for use in the high-throughput study of mitosis and other intracellular processes, as is being done with various animal cell lines.

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Acknowledgements

We are indebted to Mitsuyasu Hasebe, Yuji Hiwatashi, and other Hasebe laboratory members for all the available and shared reagents for cell biology in moss. They also have provided us valuable information regarding the techniques associated with moss culturing and imaging. We thank Yuki Nakaoka and Ken Kosetsu for developing protocols and critical reading of this chapter. Work on moss in our laboratory is supported by Human Frontier Science Program, the TORAY Science Foundation, and Grants-in-Aid for Scientific Research (15H01227, 15K14540, and 26711012; MEXT, Japan).

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Authors and Affiliations

  1. Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

    Moé Yamada & Tomohiro Miki

  2. Division of Biological Science, Graduate School of Science, Nagoya University, Room 227, Building A, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

    Gohta Goshima

Authors
  1. Moé Yamada
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  2. Tomohiro Miki
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  3. Gohta Goshima
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Corresponding author

Correspondence to Gohta Goshima .

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Editors and Affiliations

  1. Massachusetts Institute of Technolo, Cambridge, Massachusetts, USA

    Paul Chang

  2. Dept of Cell & Developmental Biolog, Vanderbilt University, Nashville, Tennessee, USA

    Ryoma Ohi

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Yamada, M., Miki, T., Goshima, G. (2016). Imaging Mitosis in the Moss Physcomitrella patens . In: Chang, P., Ohi, R. (eds) The Mitotic Spindle. Methods in Molecular Biology, vol 1413. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3542-0_17

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  • DOI: https://doi.org/10.1007/978-1-4939-3542-0_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3540-6

  • Online ISBN: 978-1-4939-3542-0

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