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In Situ Detection of Interactions Between Nuclear Envelope Proteins and Partners

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The Nuclear Envelope

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1411))

Abstract

Proximity ligation assay (PLA) appears as a quick and easy technique to visualize within fixed cells the occurrence and in situ distribution of protein complexes. PLA has been validated to detect protein-protein interactions within the nuclear compartment. Here, we describe a protocol which allows the detection of interactions between A-type nuclear lamins and either LEM-domain proteins (such as emerin, integrated within the inner nuclear membrane, and LAP2α which accumulates within the nucleoplasm) or gene regulatory factors (e.g., the transcription factor SREBP1). The distinct amounts and patterns of PLA signals obtained for various complexes highlight the pertinence of using PLA to reveal in situ where and to which extent nuclear envelope proteins bind specific partners.

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Acknowledgment

This work was supported by CNRS, University Paris Diderot Paris 7, and the Association Française contre les Myopathies.

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Authors and Affiliations

  1. Unit of Functional and Adaptive Biology (BFA) CNRS UMR 8251, Université Paris Diderot, Sorbonne Paris Cité, 4 rue Marie-Andrée Lagroua Weill-Halle, Paris Cedex 13, 75205, France

    Alice Barateau & Brigitte Buendia

Authors
  1. Alice Barateau
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  2. Brigitte Buendia
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Corresponding author

Correspondence to Brigitte Buendia .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Leicester, Leicester, United Kingdom

    Sue Shackleton

  2. Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

    Philippe Collas

  3. Wellcome Trust Centre for Cell Biol, University of Edinburgh, Edinburgh, United Kingdom

    Eric C. Schirmer

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Barateau, A., Buendia, B. (2016). In Situ Detection of Interactions Between Nuclear Envelope Proteins and Partners. In: Shackleton, S., Collas, P., Schirmer, E. (eds) The Nuclear Envelope. Methods in Molecular Biology, vol 1411. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3530-7_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3530-7_9

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3528-4

  • Online ISBN: 978-1-4939-3530-7

  • eBook Packages: Springer Protocols

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