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Quantitative Functional Morphology by Imaging Flow Cytometry

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Imaging Flow Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1389))

Abstract

This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.

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Acknowledgements

The work was supported in part by NIH S10 RR023459 grant, Harvard Pilot grant, and the Russian Foundation for Basic Research grants 13-04-40189-H and 11-04-01749a. We are also grateful to Aleksandra Gorelova (Harvard University) for help with the editing and preparation of the manuscript.

Author information

Authors and Affiliations

  1. Department of Cell Biology and Histology, M.V. Lomonosov Moscow State University, Moscow, 119992, Russia

    Ivan A. Vorobjev

  2. A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, 119992, Russia

    Ivan A. Vorobjev

  3. Cellular and Molecular Medicine Program, Boston Childrens Hospital, Boston, MA, 02115, USA

    Natasha S. Barteneva

  4. Department of Pediatrics, Harvard Medical School, Boston, MA, 02115, USA

    Natasha S. Barteneva

Authors
  1. Ivan A. Vorobjev
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  2. Natasha S. Barteneva
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Corresponding authors

Correspondence to Ivan A. Vorobjev or Natasha S. Barteneva .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. A.N. Belozersky Institute of Physico-Che, M.V. Lomonosov Moscow State University, Moscow, Russia

    Ivan A. Vorobjev

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Vorobjev, I.A., Barteneva, N.S. (2016). Quantitative Functional Morphology by Imaging Flow Cytometry. In: Barteneva, N., Vorobjev, I. (eds) Imaging Flow Cytometry. Methods in Molecular Biology, vol 1389. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3302-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-3302-0_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3300-6

  • Online ISBN: 978-1-4939-3302-0

  • eBook Packages: Springer Protocols

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