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Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells

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Lipid Signaling Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1376))

Abstract

Accumulating evidence implicates phosphoinositide 4-phosphate as a regulatory molecule in its own right recruiting specific effector proteins to cellular membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.

*These authors contributed equally to this work.

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Correspondence to Krista Rombouts Ph.D. or Vinicio Carloni M.D., Ph.D. .

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Rombouts, K., Carloni, V. (2016). Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells. In: Waugh, M. (eds) Lipid Signaling Protocols. Methods in Molecular Biology, vol 1376. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3170-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-3170-5_17

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3169-9

  • Online ISBN: 978-1-4939-3170-5

  • eBook Packages: Springer Protocols

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