Abstract
A number of model organisms have provided the basis for our understanding of the eukaryotic cell cycle. These model organisms are generally much easier to manipulate than mammalian cells and as such provide amenable tools for extensive genetic and biochemical analysis. One of the most common model organisms used to study the cell cycle is the budding yeast Saccharomyces cerevisiae. This model provides the ability to synchronise cells efficiently at different stages of the cell cycle, which in turn opens up the possibility for extensive and detailed study of mechanisms regulating the eukaryotic cell cycle. Here, we describe methods in which budding yeast cells are arrested at a particular phase of the cell cycle and then released from the block, permitting the study of molecular mechanisms that drive the progression through the cell cycle.
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Acknowledgements
We are grateful for teaching and scientific advice from Professor Karim Labib. Methods described in this chapter were extensively used in the Labib laboratory and we would like to thank members of his group, past and present who contributed to our current understanding of methods presented here. We especially thank Dr. Ben Hodgson for comments on this chapter. ASD is a recipient of a Ramon y Cajal contract and received funding from the Cantabria International Campus and via grant BFU2011-23193 from the Spanish “Ministerio de Economia y Competitividad” (co-funded by the European Regional Development Fund).
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Foltman, M., Molist, I., Sanchez-Diaz, A. (2016). Synchronization of the Budding Yeast Saccharomyces cerevisiae . In: Sanchez-Diaz, A., Perez, P. (eds) Yeast Cytokinesis. Methods in Molecular Biology, vol 1369. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3145-3_19
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DOI: https://doi.org/10.1007/978-1-4939-3145-3_19
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3144-6
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