Abstract
The budding yeast Saccharomyces cerevisiae is widely used as a model system to study the organization and function of the cytoskeleton. In the past, its small size, rounded shape, and rigid cell wall created obstacles to explore the cell biology of this model eukaryote. It is now possible to acquire and analyze high-resolution and super-resolution multidimensional images of the yeast cell. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing the cytoskeleton and interactions of the actin cytoskeleton with mitochondria in fixed yeast cells using wide-field and super-resolution fluorescence microscopy.
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Acknowledgments
This work was supported by awards from the Ellison Medical Foundation (AG-SS-2465) and the NIH (GM45735, GM45735S1, and GM096445) to L.A.P. GM45735S1 was issued from the NIH under the American Recovery and Reinvestment Act of 2009. The microscopes used for these studies were supported in part through a NIH ⁄ NCI grant (5 P30 CA13696) and obtained using funds from the NIH-NCRR (1S10OD014584) to L.A.P.
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Higuchi-Sanabria, R., Swayne, T.C., Boldogh, I.R., Pon, L.A. (2016). Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells. In: Gavin, R. (eds) Cytoskeleton Methods and Protocols. Methods in Molecular Biology, vol 1365. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3124-8_3
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DOI: https://doi.org/10.1007/978-1-4939-3124-8_3
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3123-1
Online ISBN: 978-1-4939-3124-8
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