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Import of Fluorescent RNA into Mitochondria of Living Cells

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Mitochondrial DNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1351))

Abstract

Methods of in vivo visualization and manipulation of mitochondrial genetic machinery are limited due to the need to surpass not only the cytoplasmic membrane but also two mitochondrial membranes. Here, we employ the matrix-addressing sequence of mitochondrial ribosomal 5S-rRNA (termed MAM), which is naturally imported into mammalian mitochondria, to construct an import system for in vivo targeting of mitochondrial (mt) DNA or mtRNA, in order to provide fluorescence hybridization of the desired sequences.

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Acknowledgement

This work was supported by the Czech Science Foundation grant No. P305/12/P388 to JZ and by the research projects RVO67985823 and BIOCEV—Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University (CZ.1.05/1.1.00/02.0109), the European Regional Development Fund.

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Authors and Affiliations

  1. Department No. 75, Membrane Transprot Biophysics, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1084, Prague 4, 14220, Czech Republic

    Jaroslav Zelenka & Petr Ježek Ph.D., D.Sc.

Authors
  1. Jaroslav Zelenka
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  2. Petr Ježek Ph.D., D.Sc.
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Corresponding author

Correspondence to Petr Ježek Ph.D., D.Sc. .

Editor information

Editors and Affiliations

  1. Hudson Institute of Medical Research, Clayton, Melbourne, Victoria, Australia

    Matthew McKenzie

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Zelenka, J., Ježek, P. (2016). Import of Fluorescent RNA into Mitochondria of Living Cells. In: McKenzie, M. (eds) Mitochondrial DNA. Methods in Molecular Biology, vol 1351. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3040-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3040-1_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3039-5

  • Online ISBN: 978-1-4939-3040-1

  • eBook Packages: Springer Protocols

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