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Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination

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Vaccine Technologies for Veterinary Viral Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1349))

Abstract

Particulate material is more efficient in eliciting immune responses. Here we describe the production of microspheres formed by protein muNS-Mi from avian reoviruses, loaded with foreign epitopes by means of IC-Tagging, for their use as vaccines.

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References

  1. Brandariz-Nuñez A, Menaya-Vargas R, Benavente J et al (2010) Avian reovirus muNS protein forms homo-oligomeric inclusions in a microtubule-independent fashion, which involves specific regions of its C-terminal domain. J Virol 84:4289–4301

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain

    Natalia Barreiro-Piñeiro, Rebeca Menaya-Vargas, Alberto Brandariz-Núñez, Iria Otero-Romero, Irene Lostalé-Seijo, Javier Benavente & José M. Martínez-Costas

  2. Department of Biochemistry, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Alberto Brandariz-Núñez

Authors
  1. Natalia Barreiro-Piñeiro
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  2. Rebeca Menaya-Vargas
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  3. Alberto Brandariz-Núñez
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  4. Iria Otero-Romero
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  5. Irene Lostalé-Seijo
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  6. Javier Benavente
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  7. José M. Martínez-Costas
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Corresponding author

Correspondence to José M. Martínez-Costas .

Editor information

Editors and Affiliations

  1. CISA-INIA, Valdeolmos, Madrid, Spain

    Alejandro Brun

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© 2016 Springer Science+Business Media New York

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Barreiro-Piñeiro, N. et al. (2016). Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination. In: Brun, A. (eds) Vaccine Technologies for Veterinary Viral Diseases. Methods in Molecular Biology, vol 1349. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3008-1_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3008-1_2

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3007-4

  • Online ISBN: 978-1-4939-3008-1

  • eBook Packages: Springer Protocols

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