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Regulation of TGF-β Receptors

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TGF-β Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1344))

Abstract

In cells responding to extracellular polypeptide ligands, regulatory mechanisms at the level of cell surface receptors are increasingly seen to define the nature of the ligand-induced signaling responses. Processes that govern the levels of receptors at the plasma membrane, including posttranslational modifications, are crucial to ensure receptor function and specify the downstream signals. Indeed, extracellular posttranslational modifications of the receptors help define stability and ligand binding, while intracellular modifications mediate interactions with signaling mediators and accessory proteins that help define the nature of the signaling response. The use of various molecular biology and biochemistry techniques, based on chemical crosslinking, e.g., biotin or radioactive labeling, immunofluorescence to label membrane receptors and flow cytometry, allows for quantification of changes of cell surface receptor presentation. Here, we discuss recent progress in our understanding of the regulation of TGF-β receptors, i.e., the type I (TβRI) and type II (TβRII) TGF-β receptors, and describe basic methods to identify and quantify TGF-β cell surface receptors.

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Acknowledgments

We thank Michael Kissner for discussion on the flow cytometry experiments. Research by the authors is sponsored by a Juvenile Diabetes Research Fund postdoctoral fellowship to E.B., an American Heart Association scientist development award to J.X., and NIH RO1 grants CA63101 and CA136690 to R.D.

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Authors and Affiliations

  1. Department of Cell and Tissue Biology, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Programs in Cell Biology, and Developmental and Stem Cell Biology, University of California, San Francisco, CA, USA

    Erine H. Budi & Rik Derynck

  2. Center for Craniofacial Molecular Biology, Ostrow School of Dentistry of USC, University of Southern California, Los Angeles, CA, USA

    Jian Xu

Authors
  1. Erine H. Budi
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  2. Jian Xu
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  3. Rik Derynck
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Correspondence to Rik Derynck .

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Editors and Affiliations

  1. Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

    Xin-Hua Feng

  2. Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China

    Pinglong Xu

  3. Department of Surgery, Baylor College of Medicine, Houston, Texas, USA

    Xia Lin

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Budi, E.H., Xu, J., Derynck, R. (2016). Regulation of TGF-β Receptors. In: Feng, XH., Xu, P., Lin, X. (eds) TGF-β Signaling. Methods in Molecular Biology, vol 1344. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2966-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2966-5_1

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2965-8

  • Online ISBN: 978-1-4939-2966-5

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