Abstract
Newt lens regeneration is achieved by a unique cellular regulation of transdifferentiation where the dorsal iris pigmented epithelial cells (PECs) dedifferentiate and redifferentiate into lens cells. Recent studies have shown that nuclear architecture of PECs is dynamically changed and unique epigenetic regulation in somatic nucleus is crucial in the lens transdifferentiation. Immuno-FISH, detection of protein and gene loci in nucleus, is one of the effective tools to analyze nuclear architecture of PECs. In this chapter a whole process from lentectomy to immuno-FISH is described.
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References
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Acknowledgments
This work is supported by JST PRESTO and a grant, KAKENHI (14514364), to N.M. I would like to thank Panagiotis A. Tsonis for critical reading and Rinako Maki for producing the illustrations.
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Maki, N. (2015). Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH. In: Kumar, A., Simon, A. (eds) Salamanders in Regeneration Research. Methods in Molecular Biology, vol 1290. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2495-0_6
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DOI: https://doi.org/10.1007/978-1-4939-2495-0_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2494-3
Online ISBN: 978-1-4939-2495-0
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