Abstract
Metastatic spread of cancer cells is the main cause of death of breast cancer patients. A better understanding of the molecular mechanism of cancer metastasis is essential for the development of novel and effective therapies. The biological complexity of the metastasis process requires the combination of multiple experimental systems to model distinct steps of cancer metastasis. Several animal models have been generated to mimic the process of breast cancer metastasis, with unique advantages and drawbacks of each model. In this chapter, we describe transplantable xenograft and allograft methods to introduce human or mouse breast tumor cells into mice in order to generate spontaneous and experimental metastasis.
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Acknowledgements
We thank Brian Ell, Hanqiu Zheng, and Mark Esposito for comments and suggestions for the manuscript. Research in our laboratory is supported by grants from the Brewster Foundation, the Department of Defense (BC123187) and the National Institutes of Health (R01CA134519 and R01CA141062) to Y.K. R.C. is a recipient of a DOD postdoctoral fellowship (W81XWH-11-1-0681).
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Chakrabarti, R., Kang, Y. (2015). Transplantable Mouse Tumor Models of Breast Cancer Metastasis. In: Eferl, R., Casanova, E. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 1267. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2297-0_18
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DOI: https://doi.org/10.1007/978-1-4939-2297-0_18
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