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High-Throughput Small-Molecule Screening in Caenorhabditis elegans

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Chemical Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1263))

Abstract

Chemical compounds, which modulate enzymatic activities or those which induce specific phenotypes of interest, are valuable probes to study biological phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm Caenorhabditis elegans (C. elegans) using 96- or 384-well microtiter plates.

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Acknowledgements

This work was supported by grant funding to M.P., from the NIH (DP2 OD008398), a grant from the Ellison Medical foundation (AG-NS-0928-12), an MDA Development Grant for S.R., and an NSF GRFP Fellowship for G.M.S. Strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440).

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Authors and Affiliations

  1. Department of Chemical Physiology, The Scripps Research Institute, 10550 N Torrey Pines Rd, Mail drop: MEM 268, La Jolla, CA, 92037, USA

    Sunitha Rangaraju, Gregory M. Solis & Michael Petrascheck Ph.D.

  2. Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 N Torrey Pines Rd, Mail drop: MEM 268, La Jolla, CA, 92037, USA

    Sunitha Rangaraju, Gregory M. Solis & Michael Petrascheck Ph.D.

  3. Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 N Torrey Pines Rd, Mail drop: MEM 268, La Jolla, CA, 92037, USA

    Sunitha Rangaraju, Gregory M. Solis & Michael Petrascheck Ph.D.

Authors
  1. Sunitha Rangaraju
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  2. Gregory M. Solis
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  3. Michael Petrascheck Ph.D.
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Corresponding author

Correspondence to Michael Petrascheck Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Medicine - Cardiovascular Medicine, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Jonathan E. Hempel

  2. Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Charles H. Williams

  3. Department of Medicine - Cardiovascular Medicine, Department of Pharmacology, Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Charles C. Hong

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Rangaraju, S., Solis, G.M., Petrascheck, M. (2015). High-Throughput Small-Molecule Screening in Caenorhabditis elegans . In: Hempel, J., Williams, C., Hong, C. (eds) Chemical Biology. Methods in Molecular Biology, vol 1263. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2269-7_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2269-7_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2268-0

  • Online ISBN: 978-1-4939-2269-7

  • eBook Packages: Springer Protocols

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