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Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays

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Nuclear Bodies and Noncoding RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1262))

Abstract

Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis-acting regulatory regions in the mRNA transcript that mediate direct interactions with trans-acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.

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References

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Author information

Authors and Affiliations

  1. Department of Surgery, The Jefferson Pancreas, Biliary, and Related Cancer Center, Thomas Jefferson University, 623 College Building, 1015 Walnut St., Philadelphia, PA, 19107, USA

    Joseph A. Cozzitorto, Masaya Jimbo, Saswati Chand, Fernando Blanco, Shruti Lal, Melissa Gilbert, Jordan M. Winter & Jonathan R. Brody

  2. Laboratory of Genetics, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD, 21224, USA

    Myriam Gorospe

  3. Department of Pharmacology and Experimental Therapeutics, Thomas Jefferson University, 623 College Building, 1015 Walnut St., Philadelphia, PA, 19107, USA

    Fernando Blanco

Authors
  1. Joseph A. Cozzitorto
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  2. Masaya Jimbo
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  3. Saswati Chand
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  4. Fernando Blanco
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  5. Shruti Lal
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  6. Melissa Gilbert
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  7. Jordan M. Winter
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  8. Myriam Gorospe
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  9. Jonathan R. Brody
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Corresponding author

Correspondence to Jonathan R. Brody .

Editor information

Editors and Affiliations

  1. RNA Biology Laboratory, RIKEN, Saitama, Japan

    Shinichi Nakagawa

  2. Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan

    Tetsuro Hirose

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© 2015 Springer Science+Business Media New York

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Cozzitorto, J.A. et al. (2015). Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays. In: Nakagawa, S., Hirose, T. (eds) Nuclear Bodies and Noncoding RNAs. Methods in Molecular Biology, vol 1262. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2253-6_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2253-6_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2252-9

  • Online ISBN: 978-1-4939-2253-6

  • eBook Packages: Springer Protocols

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