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Cryopreservation of Hepatocytes

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Protocols in In Vitro Hepatocyte Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1250))

Abstract

The use of cryopreserved hepatocytes has increased in the last decade due to the improvement of the freezing and thawing methods, and has even achieved acceptance by the US Food and Drug Administration for use in drug metabolizing enzyme induction studies. This chapter provides an overview of the theories behind the process of cryopreservation as well as practical advice on methods to cryopreserve hepatocytes, which retain functions similar to fresh cells after thawing. Parameters, such as cell density, cryoprotectants, freezing media, storage conditions, and thawing techniques, should be critically considered. Special emphasis is put on human hepatocytes, but information for the cryopreservation of animal hepatocytes is also described.

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Authors and Affiliations

  1. SWS, Wingertstrasse 25, 64390, Erzhausen, Germany

    Nicola J. Hewitt

  2. In Vitro ADMET Laboratories LLC, 9221 Rumsey Road Suite 8, Columbia, MD, 21045, USA

    Albert P. Li

Authors
  1. Nicola J. Hewitt
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  2. Albert P. Li
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Correspondence to Nicola J. Hewitt .

Editor information

Editors and Affiliations

  1. Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium

    Mathieu Vinken

  2. Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium

    Vera Rogiers

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Hewitt, N.J., Li, A.P. (2015). Cryopreservation of Hepatocytes. In: Vinken, M., Rogiers, V. (eds) Protocols in In Vitro Hepatocyte Research. Methods in Molecular Biology, vol 1250. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2074-7_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2074-7_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2073-0

  • Online ISBN: 978-1-4939-2074-7

  • eBook Packages: Springer Protocols

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