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Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe

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Cervical Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1249))

Abstract

Deubiquitinating enzymes (DUBs) are a novel “drug-able” target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

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Author information

Authors and Affiliations

  1. Department of Obstetrics, University of Minnesota Medical School, MMC 395 Mayo, 8395C (Campus Delivery Code), 420 Delaware Street SE, Minneapolis, MN, 55455, USA

    Colleen Rivard M.D. & Martina Bazzaro Ph.D.

  2. Department of Gynaecology, University of Minnesota Medical School, MMC 395 Mayo, 8395C (Campus Delivery Code), 420 Delaware Street SE, Minneapolis, MN, 55455, USA

    Martina Bazzaro Ph.D.

Authors
  1. Colleen Rivard M.D.
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  2. Martina Bazzaro Ph.D.
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Corresponding author

Correspondence to Colleen Rivard M.D. .

Editor information

Editors and Affiliations

  1. Touro University California, Vallejo, California, USA

    Daniel Keppler

  2. Touro University California, Vallejo, California, USA

    Athena W. Lin

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Rivard, C., Bazzaro, M. (2015). Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe. In: Keppler, D., Lin, A. (eds) Cervical Cancer. Methods in Molecular Biology, vol 1249. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2013-6_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2013-6_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2012-9

  • Online ISBN: 978-1-4939-2013-6

  • eBook Packages: Springer Protocols

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