Skip to main content

Studies of Intracellular Angiotensin II

  • Protocol
  • First Online:
Nuclear G-Protein Coupled Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1234))

Abstract

Many extracellular signaling proteins act within their cells of synthesis and/or in target cells after internalization. This type of action is called intracrine and it plays a role in diverse biological processes. The mechanisms of intracrine intracellular action are becoming clear thanks to the application of modern techniques of molecular biology. Here, progress in this area is reviewed. In particular the intracrine biology of angiotensin II is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Robertson AL Jr, Khairallah PA (1971) Angiotensin II: rapid localization in nuclei of smooth and cardiac muscle. Science 172:1138–1139

    Article  CAS  PubMed  Google Scholar 

  2. Re RN, MacPhee AA, Fallon JT (1981) Specific nuclear binding of angiotensin II by rat liver and spleen nuclei. Clin Sci (Lond) 61:245S–247S

    CAS  Google Scholar 

  3. Re R, Parab M (1984) Effect of angiotensin II on RNA synthesis by isolated nuclei. Life Sci 34:647–651

    Article  CAS  PubMed  Google Scholar 

  4. Re RN, LaBiche RA, Bryan SE (1983) Nuclear-hormone mediated changes in chromatin solubility. Biochem Biophys Res Commun 110:61–68

    Article  CAS  PubMed  Google Scholar 

  5. Re RN, Vizard DL, Brown J et al (1984) Angiotensin II receptors in chromatin fragments generated by micrococcal nuclease. Biochem Biophys Res Commun 119:220–227

    Article  CAS  PubMed  Google Scholar 

  6. Re RN, Vizard DL, Brown J et al (1984) Angiotensin II receptors in chromatin. J Hypertens Suppl 2:S271–S273

    CAS  PubMed  Google Scholar 

  7. Re R, Bryan SE (1984) Functional intracellular renin-angiotensin systems may exist in multiple tissues. Clin Exp Hypertens A 6:1739–1742

    Article  CAS  PubMed  Google Scholar 

  8. Cook JL, Zhang Z, Re RN (2001) In vitro evidence for an intracellular site of angiotensin action. Circ Res 89:1138–1146

    Article  CAS  PubMed  Google Scholar 

  9. Cook JL, Giardina JF, Zhang Z et al (2002) Intracellular angiotensin II increases the long isoform of PDGF mRNA in rat hepatoma cells. J Mol Cell Cardiol 34:1525–1537

    Article  CAS  PubMed  Google Scholar 

  10. Cook JL, Mills SJ, Naquin R et al (2006) Nuclear accumulation of the AT1 receptor in a rat vascular smooth muscle cell line: effects upon signal transduction and cellular proliferation. J Mol Cell Cardiol 40:696–707

    Article  CAS  PubMed  Google Scholar 

  11. Cook JL, Mills SJ, Naquin RT et al (2007) Cleavage of the angiotensin II type 1 receptor and nuclear accumulation of the cytoplasmic carboxy-terminal fragment. Am J Physiol Cell Physiol 292:C1313–C1322

    Article  CAS  PubMed  Google Scholar 

  12. Redding KM, Chen BL, Singh A et al (2010) Transgenic mice expressing an intracellular fluorescent fusion of angiotensin II demonstrate renal thrombotic microangiopathy and elevated blood pressure. Am J Physiol Heart Circ Physiol 298:H1807–H1818

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Cook JL, Re RN (2011) Lessons from in vitro studies and a related intracellular angiotensin II transgenic mouse model. Am J Physiol Regul Integr Comp Physiol 302:R482–R493

    Article  PubMed Central  PubMed  Google Scholar 

  14. Re RN, Cook JL (2011) Noncanonical intracrine action. J Am Soc Hypertens 5:435–448

    Article  CAS  PubMed  Google Scholar 

  15. Abadir PM, Foster DB, Crow M et al (2011) Identification and characterization of a functional mitochondrial angiotensin system. Proc Natl Acad Sci U S A 108:14849–14854

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Premer C, Lamondin C, Mitzey A et al (2013) Immunohistochemical localization of AT1a, AT1b, and AT2 angiotensin II receptor subtypes in the rat adrenal, pituitary, and brain with a perspective commentary. Int J Hypertens 2013:175428. doi:10.1155/2013/175428

    Article  PubMed Central  PubMed  Google Scholar 

  17. Cook JL, Singh A, DeHaro D et al (2011) Expression of a naturally occurring angiotensin AT(1) receptor cleavage fragment elicits caspase-activation and apoptosis. Am J Physiol Cell Physiol 301:C1175–C1185

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Tadevosyan A, Vaniotis G, Allen BG et al (2012) G protein-coupled receptor signalling in the cardiac nuclear membrane: evidence and possible roles in physiological and pathophysiological function. J Physiol 590:1313–1330

    CAS  PubMed Central  PubMed  Google Scholar 

  19. Re RN (2002) The origins of intracrine hormone action. Am J Med Sci 323:43–48

    Article  PubMed  Google Scholar 

  20. Re RN (2003) The intracrine hypothesis and intracellular peptide hormone action. Bioessays 25:401–409

    Article  CAS  PubMed  Google Scholar 

  21. Re RN, Cook JL (2006) The intracrine hypothesis: an update. Regul Pept 133:1–9

    Article  CAS  PubMed  Google Scholar 

  22. Re RN, Cook JL (2008) The basis of an intracrine pharmacology. J Clin Pharmacol 48:344–350

    Article  CAS  PubMed  Google Scholar 

  23. Re RN, Cook JL (2009) Senescence, apoptosis, and stem cell biology: the rationale for an expanded view of intracrine action. Am J Physiol Heart Circ Physiol 297:H893–H901

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. Re RN, Cook JL (2010) The mitochondrial component of intracrine action. Am J Physiol Heart Circ Physiol 299:H577–H583

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  25. Re RN (2011) Lysosomal action of intracrine angiotensin II. Focus on “Intracellular angiotensin II activates rat myometrium”. Am J Physiol Cell Physiol 301:C553–C554

    Article  CAS  PubMed  Google Scholar 

  26. Kumar R, Thomas CM, Yong QC et al (2012) The intracrine renin-angiotensin system. Clin Sci (Lond) 123:273–284

    Article  CAS  Google Scholar 

  27. Re RN (2013) Could intracrine biology play a role in the pathogenesis of transmissible spongiform encephalopathies, Alzheimer’s Disease, and other neurodegenerative diseases? Am J Med Sci 347:312–320

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Richard N. Re .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Re, R.N., Cook, J.L. (2015). Studies of Intracellular Angiotensin II. In: Allen, B., Hébert, T. (eds) Nuclear G-Protein Coupled Receptors. Methods in Molecular Biology, vol 1234. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1755-6_1

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1755-6_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1754-9

  • Online ISBN: 978-1-4939-1755-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics