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Mouse Models of Osteoarthritis: Surgical Model of Posttraumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus

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Osteoporosis and Osteoarthritis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1226))

Abstract

The surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA). The DMM model mimics clinical meniscal injury, a known predisposing factor for the development of human OA, and permits the study of structural and biological changes over the course of the disease. In addition, when applied to genetically modified or engineered mouse models, this surgical procedure permits dissection of the relative contribution of a given gene to OA initiation and/or progression. This chapter describes the requirements for the surgical induction of OA in mouse models, and provides guidelines and tools for the subsequent histological, immunohistochemical, and molecular analyses. Methods for the assessment of the contributions of selected genes in genetically modified strains are also provided.

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Acknowledgements

Research related to this topic is supported by National Institutes of Health grants R01-AG-022021 and RC4-AR060546.

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Authors and Affiliations

  1. Tissue Engineering Regeneration and Repair Program, Research Division, The Hospital for Special Surgery, Caspary Research Building, 5th Floor, 535 East 70th Street, New York, NY, 10021, USA

    Kirsty L. Culley Ph.D., Cecilia L. Dragomir M.D., Jun Chang B.S., Elisabeth B. Wondimu, Jonathan Coico B.S., Miguel Otero Ph.D. & Mary B. Goldring Ph.D.

  2. Weill Cornell Medical College, New York, NY, USA

    Kirsty L. Culley Ph.D., Jun Chang B.S., Elisabeth B. Wondimu, Jonathan Coico B.S., Miguel Otero Ph.D. & Mary B. Goldring Ph.D.

  3. Breakthrough Breast Cancer Research Unit, King’s College London School of Medicine, Guy’s Hospital, London, UK

    Darren A. Plumb Ph.D.

Authors
  1. Kirsty L. Culley Ph.D.
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  2. Cecilia L. Dragomir M.D.
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  3. Jun Chang B.S.
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  4. Elisabeth B. Wondimu
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  5. Jonathan Coico B.S.
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  6. Darren A. Plumb Ph.D.
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  7. Miguel Otero Ph.D.
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  8. Mary B. Goldring Ph.D.
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Corresponding authors

Correspondence to Kirsty L. Culley Ph.D. or Mary B. Goldring Ph.D. .

Editor information

Editors and Affiliations

  1. Mayo Clinic, Rochester, Minnesota, USA

    Jennifer J. Westendorf

  2. Mayo Clinic, Rochester, Minnesota, USA

    Andre J. van Wijnen

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Culley, K.L. et al. (2015). Mouse Models of Osteoarthritis: Surgical Model of Posttraumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus. In: Westendorf, J., van Wijnen, A. (eds) Osteoporosis and Osteoarthritis. Methods in Molecular Biology, vol 1226. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1619-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-1619-1_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1618-4

  • Online ISBN: 978-1-4939-1619-1

  • eBook Packages: Springer Protocols

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