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miRNAs Expression Profile in Zebrafish Developing Vessels

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Vascular Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1214))

Abstract

In this chapter we will describe in detail a method to identify the expression profile of miRNAs in developing vessels in zebrafish embryonic development using Illumina deep sequencing strategy. We will describe how to obtain RNA from FACS-sorted primary endothelial cells from growing vessels at early stages of development and how to prepare high-quality small RNA libraries using the TruSeq small RNA strategy for Illumina Hi-Seq machine. This methodology can be applied to discover and profile all forms of small noncoding RNA, including novel miRNA and sequence variants as well as quantification of miRNAs differentially expressed in endothelial cells during angiogenesis.

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Authors and Affiliations

  1. Yale Cardiovascular Research Center, Internal Medicine, Yale University, New Haven, CT, USA

    Emma Ristori & Stefania Nicoli

Authors
  1. Emma Ristori
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  2. Stefania Nicoli
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Corresponding author

Correspondence to Stefania Nicoli .

Editor information

Editors and Affiliations

  1. University of Bari Aldo Moro, Bari, Italy

    Domenico Ribatti

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© 2015 Springer Science+Business Media New York

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Ristori, E., Nicoli, S. (2015). miRNAs Expression Profile in Zebrafish Developing Vessels. In: Ribatti, D. (eds) Vascular Morphogenesis. Methods in Molecular Biology, vol 1214. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1462-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1462-3_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1461-6

  • Online ISBN: 978-1-4939-1462-3

  • eBook Packages: Springer Protocols

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