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The In Vivo Evaluation of the Therapeutic Potential of Human Adipose Tissue-Derived Mesenchymal Stem Cells for Acute Liver Disease

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Animal Models for Stem Cell Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1213))

Abstract

Mesenchymal stem cells (MSCs) have emerged as an attractive candidate for cell therapy applications. In the prior decade, many animal studies have demonstrated that MSCs are therapeutically beneficial for the treatment of liver disease. The carbon tetrachloride (CCl4)-induced acute hepatitis model has been the most widely used model in these studies. Our group has utilized the CCl4-induced mouse hepatitis model to study the therapeutic potential of human adipose tissue-derived MSCs (hADSCs). We have demonstrated that systemically administered hADSCs engrafted into the damaged liver and promoted tissue repair. This phenomenon likely reflected the paracrine effects of the administered hADSCs. In this chapter, we describe a method to evaluate the therapeutic efficacy of the systemic administration of hADSCs in the CCl4-induced mouse model of acute hepatitis.

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Acknowledgements

We would like to thank Dr. Nobuyoshi Kosaka for his valuable advice. This work was supported in part by a Grant-in-Aid for the Third-Term Comprehensive 10-Year Strategy for Cancer Control, a Grant-in-Aid for Scientific Research on Priority Areas Cancer from the Ministry of Education, Culture, Sports, Science, and Technology, and the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NiBio), and the Japan Society for the Promotion of Science (JSPS) through the “Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)” initiated by the Council for Science and Technology Policy (CSTP), and Grant-in-Aid for Scientific Research on Innovative Areas (“functional machinery for non-coding RNAs”) from the Japanese Ministry of Education, Culture, Sports, Science, and Technology.

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Authors and Affiliations

  1. Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan

    Takeshi Katsuda, Hayato Kurata, Rie Tamai, Agnieszka Banas & Takahiro Ochiya

  2. Regenerative Medicine Research and Planning Division, Rohto Pharmaceutical Co., LTD., Tokyo Office 20th Floor Shiodome Building, 1-2-20 Kaigan, Minato-ku, Tokyo, 105-0022, Japan

    Hayato Kurata, Rie Tamai & Tsuyoshi Ishii

  3. Department of Genomic Pathology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan

    Shumpei Ishikawa

Authors
  1. Takeshi Katsuda
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  2. Hayato Kurata
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  3. Rie Tamai
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  4. Agnieszka Banas
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  5. Tsuyoshi Ishii
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  6. Shumpei Ishikawa
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  7. Takahiro Ochiya
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Corresponding author

Correspondence to Takahiro Ochiya .

Editor information

Editors and Affiliations

  1. Department of Surgery, Unviersity of Leipzig, Germany

    Bruno Christ

  2. Department of Surgery, University of Leipzig, Germany

    Jana Oerlecke

  3. Department of Surgery, University of Leipzig, Germany

    Peggy Stock

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© 2014 Springer Science+Business Media New York

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Katsuda, T. et al. (2014). The In Vivo Evaluation of the Therapeutic Potential of Human Adipose Tissue-Derived Mesenchymal Stem Cells for Acute Liver Disease. In: Christ, B., Oerlecke, J., Stock, P. (eds) Animal Models for Stem Cell Therapy. Methods in Molecular Biology, vol 1213. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1453-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-1453-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1452-4

  • Online ISBN: 978-1-4939-1453-1

  • eBook Packages: Springer Protocols

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