Abstract
High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital read-out of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe an RNA-seq approach that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5′ end of all Leishmania (and other trypanosomatid) mRNAs.
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Haydock, A., Terrao, M., Sekar, A., Ramasamy, G., Baugh, L., Myler, P.J. (2015). RNA-Seq Approaches for Determining mRNA Abundance in Leishmania . In: Peacock, C. (eds) Parasite Genomics Protocols. Methods in Molecular Biology, vol 1201. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1438-8_12
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DOI: https://doi.org/10.1007/978-1-4939-1438-8_12
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