Abstract
Regulatory B cells (Bregs) have been shown to play a role in the suppression of a wide variety of immune responses. For the study of autoimmune inflammation, experimental models of arthritis have acted as a valuable tool in understanding the function of Bregs in maintaining immune homeostasis. In this chapter, we will focus on the study of transitional-2 marginal zone precursor Bregs (T2-MZPs) in the context of two experimental arthritis models: antigen-induced arthritis (AIA) and collagen-induced arthritis (CIA). We will specifically focus on how to induce arthritis as well as methods for the isolation and functional study of Bregs both in vitro and in vivo.
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Acknowledgments
This work is funded by an Arthritis Research UK program grant (MP/17707) awarded to Claudia Mauri. Elizabeth Rosser is funded by an Arthritis Research UK PhD studentship (MP/19314). We would like to thank Dr. Paul Blair for his constructive criticisms on the manuscript.
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Rosser, E.C., Mauri, C. (2014). Regulatory B Cells in Experimental Mouse Models of Arthritis. In: Vitale, G., Mion, F. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 1190. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1161-5_13
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DOI: https://doi.org/10.1007/978-1-4939-1161-5_13
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