Abstract
In recent years, microfluidic devices have become widely used in biology, and with the advantage of requiring low sample volumes, enables previously technologically infeasible experiments in hematopoietic stem cell (HSC) research. Here, we introduce a microfluidic device to investigate dynamic interactions between HSC and model niches in vitro. The device comprises a pneumatic valve which enables the culturing of different types of niche cells in different parts of the same device. Single HSCs can then be injected into the microfluidic device, manipulated, and placed onto different niches within the same device as controlled by the user. Here, we describe the device fabrication method, the HSC collection methodology, and the operational procedure for the device.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Le HP (1998) Progress and trends in ink-jet printing technology. J Imaging Sci Tech 42:49–62
Xia YN, Whitesides GM (1998) Soft lithography. Annu Rev Mater Sci 28:153–184
Mark D, Haeberle S, Roth G et al (2010) Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chem Soc Rev 39:1153–1182
Unger MA, Chou HP, Thorsen T et al (2000) Monolithic microfabricated valves and pumps by multilayer soft lithography. Science 288:113–116
Young EW, Berthier E, Guckenberger DJ et al (2011) Rapid prototyping of arrayed microfluidic systems in polystyrene for cell-based assays. Anal Chem 83:1408–1417
Martinez AW, Phillips ST, Whitesides GM et al (2010) Diagnostics for the developing world: microfluidic paper-based analytical devices. Anal Chem 82:3–10
Whitesides GM (2006) The origins and the future of microfluidics. Nature 442:368–373
Wu HW, Hsu RC, Lin CC, Hwang SM, Lee GB (2010) An integrated microfluidic system for isolation, counting, and sorting of hematopoietic stem cells. Biomicrofluidics 4(2), pii:024112
Schirhagl R, Fuereder I, Hall EW et al (2011) Microfluidic purification and analysis of hematopoietic stem cells from bone marrow. Lab Chip 11:3130–3135
Lecault V, Vaninsberghe M, Sekulovic S et al (2011) High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays. Nat Methods 8:581–586
Dykstra B, Ramunas J, Kent D et al (2006) High-resolution video monitoring of hematopoietic stem cells cultured in single-cell arrays identifies new features of self-renewal. Proc Natl Acad Sci U S A 103:8185–8190
Kobel SA, Burri O, Griffa A et al (2012) Automated analysis of single stem cells in microfluidic traps. Lab Chip 12:2843–2849
Faley SL, Copland M, Wlodkowic D et al (2009) Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells. Lab Chip 9:2659–2664
Glotzbach JP, Januszyk M, Vial IN et al (2011) An information theoretic, microfluidic-based single cell analysis permits identification of subpopulations among putatively homogeneous stem cells. Plos One 6:e21211
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Ahn, B., Wang, Z., Archer, D.R., Lam, W.A. (2014). Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level. In: Bunting, K., Qu, CK. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology, vol 1185. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1133-2_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1133-2_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1132-5
Online ISBN: 978-1-4939-1133-2
eBook Packages: Springer Protocols