Abstract
In recent years, an enormous progress has been made in applied genomics leading to identification and isolation of novel cDNAs. However, most attempts result in the acquisition of transcribed sequences that represent only a part of the mRNA’s complete sequence. Rapid Amplification of cDNA Ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. Since the first report of this technique, many significant improvements have been made on the basic approach. This chapter describes the most recent update of the relatively simple and versatile classic RACE protocol.
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References
Yeku O, Frohman MA (2011) Rapid amplification of cDNA ends (RACE). Methods Mol Biol 703: 107–122
Frohman MA, Dush MK, Martin GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci U S A 85: 8998–9002
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Zismann, V., Nourbakhsh, M. (2014). Rapid Mapping of RNA 3′ and 5′ Ends. In: Alvarez, M., Nourbakhsh, M. (eds) RNA Mapping. Methods in Molecular Biology, vol 1182. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1062-5_2
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DOI: https://doi.org/10.1007/978-1-4939-1062-5_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1061-8
Online ISBN: 978-1-4939-1062-5
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