Skip to main content

Processing the Lymph Node Biopsy

  • Protocol
  • First Online:
Histopathology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1180))

Abstract

The testing modalities available and necessary to precisely diagnose lymphadenopathies, lymphadenitides, and lymphomas are numerous. However, to much chagrin, the tissue samples submitted for evaluation are frequently limited in size. Consequently, the triage of each individual lymph node sample with judicious apportionment of tissue for diagnostic testing has become of paramount importance. We describe a practical approach to processing the lymph node specimen to maximize the diagnostic utility of the sample provided.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Ioachim HL, Medeiros LJ (2009) Ioachim’s lymph node pathology, 4th edn. Lippincott Williams & Wilkins, Philadelphia

    Google Scholar 

  2. Butler JJ (1969) Non-neoplastic lesions of lymph nodes of man to be differentiated from lymphomas. NCI Monogr 32:233–255

    CAS  Google Scholar 

  3. Grizzle WE, Fredenburgh JL, Myers RB (2008) Fixation of tissues. In: Bancroft JD, Gamble M (eds) Theory and practice of histological techniques, 6th edn. Churchill Livingstone/Elsevier, Philadelphia, pp 53–74

    Chapter  Google Scholar 

  4. Brynes RK (2010) LAC+USC Medical Center, clinical hematology laboratory, procedure manual. Los Angeles

    Google Scholar 

  5. Swerdlow SH (1992) The multiparameter approach to lymph node diagnosis. In: Biopsy interpretation of lymph nodes. Raven Press, New York, pp 1–38

    Google Scholar 

  6. Hussong JW, Arber DA, Bradley KT et al (2013) Protocol for the examination of specimens from patients with non-Hodgkin lymphoma/lymphoid neoplasms. College of American Pathologists, Cancer protocols. http://www.cap.org/apps/docs/committees/cancer/cancer_protocols/2013/NonHodgkinLymph_13protocol_3200.pdf. Accessed 29 Oct 2013

  7. Banks PM (2001) Technical factors in the preparation and evaluation of lymph node biopsies. In: Knowles DM (ed) Neoplastic hematopathology, 2nd edn. Lippincott Williams & Wilkins, Philadelphia, pp 467–482

    Google Scholar 

  8. Beard C, Nabers K, Bowling MC et al (1985) Achieving technical excellence in lymph node specimens: an update. Lab Med 16: 468–475

    Google Scholar 

  9. Kumar GL, Kiernan JA, et al (2010) Pathology education guide: special stains and H&E, 2nd edn. DAKO education guides. http://www.dako.com/08066_special_stains_eduguide.pdf. Accessed 29 Oct 2013

  10. Bonds LA, Barnes P, Foucar K et al (2005) Acetic acid-zinc-formalin: a safe alternative to B5 fixative. Am J Clin Pathol 124:205–211

    Article  CAS  PubMed  Google Scholar 

  11. Gorczyca W (2006) Flow cytometry in neoplastic hematology. Informa UK Ltd, London

    Google Scholar 

  12. Sun W (2010) Nucleic extraction and amplification. In: Grody WW (ed) Molecular diagnostics techniques and applications for the clinical laboratory. Elsevier, San Diego, pp 35–47

    Google Scholar 

  13. Evers DL, Fowler CB, Cunningham BR et al (2011) The effect of formaldehyde fixation on RNA—optimization of formaldehyde adduct removal. J Mol Diagn 13(3):282–288

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Tanapatchaiyapong P (1972) A modification of the lymph node imprint technic. Am J Clin Pathol 58(4):431–433

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Imran N. Siddiqi M.D. .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media, New York

About this protocol

Cite this protocol

Loo, E., Siddiqi, I.N. (2014). Processing the Lymph Node Biopsy. In: Day, C. (eds) Histopathology. Methods in Molecular Biology, vol 1180. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1050-2_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1050-2_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1049-6

  • Online ISBN: 978-1-4939-1050-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics