Abstract
The testing modalities available and necessary to precisely diagnose lymphadenopathies, lymphadenitides, and lymphomas are numerous. However, to much chagrin, the tissue samples submitted for evaluation are frequently limited in size. Consequently, the triage of each individual lymph node sample with judicious apportionment of tissue for diagnostic testing has become of paramount importance. We describe a practical approach to processing the lymph node specimen to maximize the diagnostic utility of the sample provided.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ioachim HL, Medeiros LJ (2009) Ioachim’s lymph node pathology, 4th edn. Lippincott Williams & Wilkins, Philadelphia
Butler JJ (1969) Non-neoplastic lesions of lymph nodes of man to be differentiated from lymphomas. NCI Monogr 32:233–255
Grizzle WE, Fredenburgh JL, Myers RB (2008) Fixation of tissues. In: Bancroft JD, Gamble M (eds) Theory and practice of histological techniques, 6th edn. Churchill Livingstone/Elsevier, Philadelphia, pp 53–74
Brynes RK (2010) LAC+USC Medical Center, clinical hematology laboratory, procedure manual. Los Angeles
Swerdlow SH (1992) The multiparameter approach to lymph node diagnosis. In: Biopsy interpretation of lymph nodes. Raven Press, New York, pp 1–38
Hussong JW, Arber DA, Bradley KT et al (2013) Protocol for the examination of specimens from patients with non-Hodgkin lymphoma/lymphoid neoplasms. College of American Pathologists, Cancer protocols. http://www.cap.org/apps/docs/committees/cancer/cancer_protocols/2013/NonHodgkinLymph_13protocol_3200.pdf. Accessed 29 Oct 2013
Banks PM (2001) Technical factors in the preparation and evaluation of lymph node biopsies. In: Knowles DM (ed) Neoplastic hematopathology, 2nd edn. Lippincott Williams & Wilkins, Philadelphia, pp 467–482
Beard C, Nabers K, Bowling MC et al (1985) Achieving technical excellence in lymph node specimens: an update. Lab Med 16: 468–475
Kumar GL, Kiernan JA, et al (2010) Pathology education guide: special stains and H&E, 2nd edn. DAKO education guides. http://www.dako.com/08066_special_stains_eduguide.pdf. Accessed 29 Oct 2013
Bonds LA, Barnes P, Foucar K et al (2005) Acetic acid-zinc-formalin: a safe alternative to B5 fixative. Am J Clin Pathol 124:205–211
Gorczyca W (2006) Flow cytometry in neoplastic hematology. Informa UK Ltd, London
Sun W (2010) Nucleic extraction and amplification. In: Grody WW (ed) Molecular diagnostics techniques and applications for the clinical laboratory. Elsevier, San Diego, pp 35–47
Evers DL, Fowler CB, Cunningham BR et al (2011) The effect of formaldehyde fixation on RNA—optimization of formaldehyde adduct removal. J Mol Diagn 13(3):282–288
Tanapatchaiyapong P (1972) A modification of the lymph node imprint technic. Am J Clin Pathol 58(4):431–433
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Loo, E., Siddiqi, I.N. (2014). Processing the Lymph Node Biopsy. In: Day, C. (eds) Histopathology. Methods in Molecular Biology, vol 1180. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1050-2_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1050-2_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1049-6
Online ISBN: 978-1-4939-1050-2
eBook Packages: Springer Protocols