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Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network

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Exocytosis and Endocytosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1174))

Abstract

The plant endomembrane trafficking system is a highly complex set of processes. This complexity presents a challenge for its study. Classical plant genetics often struggles with loss-of-function lethality and gene redundancy. Chemical genomics allows overcoming many of these issues by using small molecules of natural or synthetic origin to inhibit specific trafficking proteins thereby affecting the processes in a tunable and reversible manner. Bioactive chemicals identified by high-throughput phenotype screens must be characterized in detail starting with understanding of the specific trafficking pathways affected. Here, we describe approaches to characterize bioactive compounds that perturb vesicle trafficking. This should equip researchers with practical knowledge on how to identify endomembrane-specific trafficking pathways that may be perturbed by specific compounds and will help to eventually identify molecular targets for these small molecules.

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Acknowledgements

This work was supported by Fondecyt 1120289 and FONDEF IDeA CA12I10206.

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Authors and Affiliations

  1. Plant Molecular Biology Laboratory, Department of Biology, University of Chile, Las Palmeras 3425, 7800003, Santiago, Chile

    Cecilia Rodriguez-Furlán & Lorena Norambuena

  2. Center for Plant Cell Biology & Department of Botany and Plant Sciences, University of California, Riverside, CA, USA

    Glenn R. Hicks

Authors
  1. Cecilia Rodriguez-Furlán
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  2. Glenn R. Hicks
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  3. Lorena Norambuena
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Corresponding author

Correspondence to Lorena Norambuena .

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Editors and Affiliations

  1. Department of Human and Molecular Genetics, VCU Institute of Molecular Medicine, Virginia Commonwealth University, Richmond, Virginia, USA

    Andrei I. Ivanov

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Rodriguez-Furlán, C., Hicks, G.R., Norambuena, L. (2014). Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network. In: Ivanov, A. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 1174. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0944-5_22

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  • DOI: https://doi.org/10.1007/978-1-4939-0944-5_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0943-8

  • Online ISBN: 978-1-4939-0944-5

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