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Enzymatic Synthesis and Purification of a Defined RIG-I Ligand

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Innate DNA and RNA Recognition

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1169))

Abstract

Receptor-based nucleic acid sensing constitutes one of the most fundamental mechanisms of our innate immune system to sense viral infection. RIG-I is a cytosolic RNA helicase that senses the presence of 5′ triphosphate RNA species, a common feature of many negative strand RNA viruses. We here describe a protocol to enzymatically synthesize and to purify a defined RIG-I ligand that can be used to study RIG-I activation in vitro and in vivo.

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Acknowledgments

G.H. and V.H. are supported by the excellence cluster ImmunoSensation. M.S., G.H., and V.H. are supported by grants from the German Research Foundation (SFB704 to V.H. and G.H., SFB670 to M.S., G.H., and V.H., DFG Research Grants Program SCHL 1930/1-1 to M.S.) and the European Research Council (ERC 243046 to V.H.).

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Authors and Affiliations

  1. Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany

    Marion Goldeck, Martin Schlee & Gunther Hartmann

  2. Institute of Molecular Medicine, University Hospital, University of Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany

    Veit Hornung

Authors
  1. Marion Goldeck
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  2. Martin Schlee
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  3. Gunther Hartmann
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  4. Veit Hornung
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Corresponding author

Correspondence to Veit Hornung .

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Editors and Affiliations

  1. Ludwig Maximilians Universität München, Medizinische Klinik und Poliklinik IV, München, Germany

    Hans-Joachim Anders

  2. Helmholtz Zentrum München German Researc, Institute for Diabetes and Regeneration, Munich, Germany

    Adriana Migliorini

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Goldeck, M., Schlee, M., Hartmann, G., Hornung, V. (2014). Enzymatic Synthesis and Purification of a Defined RIG-I Ligand. In: Anders, HJ., Migliorini, A. (eds) Innate DNA and RNA Recognition. Methods in Molecular Biology, vol 1169. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0882-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-0882-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0881-3

  • Online ISBN: 978-1-4939-0882-0

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