Abstract
Lipidomic analysis using electrospray ionization triple quadrupole mass spectrometry can be employed to monitor lipid changes that occur during cold and freezing stress of plants. Here we describe the analysis of Arabidopsis thaliana polar glycerolipids with normal and oxidized acyl chains, sampled during cold and freezing treatments. Mass spectral data are processed using the online capabilities of LipidomeDB Data Calculation Environment.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Gilmour SJ, Hajela RK, Thomashow MF (1988) Cold acclimation in Arabidopsis thaliana. Plant Physiol 87:745–750
Uemura M, Joseph RA, Steponkus PL (1995) Cold acclimation of Arabidopsis thaliana (effect on plasma membrane lipid composition and freeze-induced lesions). Plant Physiol 109:15–30
Welti R, Li W, Li M, Sang Y, Biesiada H, Zhou HE, Rajashekar CB, Williams TD, Wang X (2002) Profiling membrane lipids in plant stress responses. Role of phospholipase Dα in freezing-induced lipid changes in Arabidopsis. J Biol Chem 277:31994–32002
Li W, Wang R, Li M, Li L, Wang C, Welti R, Wang X (2008) Differential degradation of extraplastidic and plastidic lipids during freezing and post-freezing recovery in Arabidopsis thaliana. J Biol Chem 283:461–468
Degenkolbe T, Giavalisco P, Zuther E, Seiwert B, Hincha DK, Willmitzer L (2012) Differential remodeling of the lipidome during cold acclimation in natural accessions of Arabidopsis thaliana. Plant J 72:972–982
Chen M, Thelen JJ (2013) ACYL-LIPID DESATURASE2 is required for chilling and freezing tolerance in Arabidopsis. Plant Cell 25:1430–1444
Routaboul JM, Fischer SF, Browse J (2000) Trienoic fatty acids are required to maintain chloroplast function at low temperatures. Plant Physiol 124:1697–1705
Moellering ER, Muthan B, Benning C (2010) Freezing tolerance in plants requires lipid remodeling at the outer chloroplast membrane. Science 330:226–228
Takami T, Shibata M, Kobayashi Y, Shikanai T (2010) De novo biosynthesis of fatty acids plays critical roles in the response of the photosynthetic machinery to low temperature in Arabidopsis. Plant Cell Physiol 51:1265–1275
Li W, Li M, Zhang W, Welti R, Wang X (2004) The plasma membrane-bound PHOSPHOLIPASE Dδ enhances freezing tolerance in Arabidopsis thaliana. Nat Biotechnol 22:427–433
Chen QF, Xiao S, Chye ML (2008) Over-expression of the Arabidopsis 10-kilodalton acyl-coenzyme A-binding protein ACBP6 enhances freezing tolerance. Plant Physiol 148:304–315
Burgos A, Szymanski J, Seiwert B, Degenkolbe T, Hannah MA, Giavalisco P, Willmitzer L (2011) Analysis of short-term changes in the Arabidopsis thaliana glycerolipidome in response to temperature and light. Plant J 66:656–668
Vu HS, Tamura P, Galeva NA, Chaturvedi R, Roth MR, Williams TD, Wang X, Shah J, Welti R (2012) Direct infusion mass spectrometry of oxylipin-containing Arabidopsis membrane lipids reveals varied patterns in different stress responses. Plant Physiol 158:324–339
Shiva S, Vu HS, Roth MR, Zhou Z, Marepally SR, Nune DS, Lushington GH, Visvanathan M, Welti R (2013) Lipidomic analysis of plant membrane lipids by direct infusion tandem mass spectrometry. Meth Mol Biol 1009:79–91
Zhou Z, Marepally SR, Nune DS, Pallakollu P, Ragan G, Roth MR, Wang L, Lushington GH, Visvanathan M, Welti R (2011) LipidomeDB data calculation environment: online processing of direct-infusion mass spectral data for lipid profiles. Lipids 46:879–884
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Ames BN (1966) Assay of inorganic phosphate, total phosphate and phosphatases. In: Neufeld E, Ginsburg V (eds) Methods in enzymology, vol. VIII: complex carbohydrates. Academic Press, New York, NY, pp 115–118
Acknowledgements
The authors would like to thank lab members Mary Roth, Pamela Tamura, Thilani Samarakoon, Sam Honey, Drew Roach, and Kaleb Lowe for their contributions to plant stress experiments in our laboratory. This work was funded by National Science Foundation MCB 0920663. Contribution no. 14-036-J from the Kansas Agricultural Experiment Station.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Vu, H.S., Shiva, S., Hall, A.S., Welti, R. (2014). A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 1166. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0844-8_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0844-8_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0843-1
Online ISBN: 978-1-4939-0844-8
eBook Packages: Springer Protocols