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Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

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Plant Cold Acclimation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1166))

Abstract

A cryo-scanning electron microscope (cryo-SEM) is a valuable tool for observing bulk frozen samples to monitor freezing responses of plant tissues and cells. Here, essential processes of a cryo-SEM to observe freezing behaviors of plant tissue cells are described.

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Acknowledgments

One of authors (S.F.) sincerely appreciates the strong support by JEOL Co. Ltd. for improvement and development of a cryo-SEM since my start of studies using a cryo-SEM in 1974. The authors also appreciate the excellent works by Mr. K. Shinbori, Institute of Low Temperature Science, Hokkaido University, for making many apparatuses of a cryo-SEM.

Author information

Authors and Affiliations

  1. Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Japan

    Seizo Fujikawa & Keita Endoh

Authors
  1. Seizo Fujikawa
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  2. Keita Endoh
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Corresponding author

Correspondence to Seizo Fujikawa .

Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Dirk K. Hincha

  2. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Ellen Zuther

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Fujikawa, S., Endoh, K. (2014). Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 1166. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0844-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-0844-8_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0843-1

  • Online ISBN: 978-1-4939-0844-8

  • eBook Packages: Springer Protocols

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