Abstract
Several techniques allow the detection of protein–protein interactions. In vivo co-immunoprecipitation (Co-IP) studies are an important complement to other commonly used techniques such as yeast two-hybrid or fluorescence complementation, as they reveal interactions between functional proteins at physiological relevant concentrations. Here, we describe an in vivo Co-IP approach using either GFP affinity matrix or specific antibodies to purify proteins of interests and their interacting partners.
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Acknowledgements
This work was supported by the DFG (LA2633-1/2) and the Max Planck Society (MPG)—Chemical Genomics Centre (CGC) through its supporting companies AstraZeneca, Bayer CropScience, Bayer Healthcare, Boehringer-Ingelheim, and Merck-Serono.
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Speth, C., Toledo-Filho, L.A.A., Laubinger, S. (2014). Immunoprecipitation-Based Analysis of Protein–Protein Interactions. In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0700-7_11
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DOI: https://doi.org/10.1007/978-1-4939-0700-7_11
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