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Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells

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Mitosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1136))

Abstract

Factors that influence the orientation of the mitotic spindle are important for the maintenance of stem cell populations and in cancer development. However, screening for these factors requires rapid quantification of alterations of the angle of the mitotic spindle in cultured cell lines. Here we describe a method to image mitotic cells and rapidly score the angle of the mitotic spindle using a simple MATLAB application to analyze a stack of Z-images.

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References

  1. Thaiparambil JT, Eggers CM, Marcus AI (2012) AMPK regulates mitotic spindle orientation through phosphorylation of myosin regulatory light chain. Mol Cell Biol 32(16):3203–3217

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Toyoshima F et al (2007) PtdIns(3,4,5)P3 regulates spindle orientation in adherent cells. Dev Cell 13(6):796–811

    Article  CAS  PubMed  Google Scholar 

  3. Fink J et al (2011) External forces control mitotic spindle positioning. Nat Cell Biol 13(7): 771–778

    Article  CAS  PubMed  Google Scholar 

  4. Kiyomitsu T, Cheeseman IM (2012) Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation. Nat Cell Biol 14(3): 311–317

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  5. Godin JD et al (2010) Huntingtin is required for mitotic spindle orientation and mammalian neurogenesis. Neuron 67(3): 392–406

    Article  CAS  PubMed  Google Scholar 

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Acknowledgement

This work was supported by NIH grant GM69429 to L.W.

Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Washington, Seattle, WA, USA

    Justin Decarreau, Jonathan Driver & Charles Asbury

  2. Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA, 98195-7290, USA

    Linda Wordeman

Authors
  1. Justin Decarreau
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  2. Jonathan Driver
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  3. Charles Asbury
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  4. Linda Wordeman
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Corresponding author

Correspondence to Linda Wordeman .

Editor information

Editors and Affiliations

  1. Dept Physiology Biophysics, Albert Einstein College of Medicine, Bronx, New York, USA

    David J. Sharp

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© 2014 Springer Science+Business Media New York

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Decarreau, J., Driver, J., Asbury, C., Wordeman, L. (2014). Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells. In: Sharp, D. (eds) Mitosis. Methods in Molecular Biology, vol 1136. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0329-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-0329-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0328-3

  • Online ISBN: 978-1-4939-0329-0

  • eBook Packages: Springer Protocols

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