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The Response of Germfree Rats to Dietary Cholesterol

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Germ-Free Biology Experimental and Clinical Aspects

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 3))

Abstract

Male germfree (G.F.) and conventional (Conv.) rats 90 to 120 days of age were fed purified diets (1) (casein-rice starch with 5% corn oil) containing 0%, 0.05%, 0.1% and 0.5% cholesterol. After a minimum of two weeks, the animals were sacrificed. Serum, liver, and intestinal tract total cholesterol were determined by a modification of the FeCl3 procedure of Caraway (2).

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References

  1. Wostmann, B.S., and T. F. Kellogg. Purified starch-casein diet for nutritional research with germfree rats. J. Lab. Animal Care. 17: 589. (1967)

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  2. Caraway, W.T. Microchemical Methods of Blood Analysis pp. 82–85 Charles C. Thomas, Springfield, Illinois, 1960.

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  3. Kellogg, Thomas F. and Bernard S. Wostmann. The Metabolism of Lipids in Germfree Animals, in The Germfree Animal in Research. M.E. Coates Ed. Academic Press, London, 1968.

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Author information

Authors and Affiliations

  1. Department of Microbiology, University of Notre Dame, Notre Dame, Indiana, USA

    Thomas Kellogg & Bernard Wostmann

Authors
  1. Thomas Kellogg
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  2. Bernard Wostmann
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Editor information

Editors and Affiliations

  1. Roswell Park Memorial Institute, New York State Department of Health, State University of New York, Buffalo, New York, USA

    Edwin A. Mirand

  2. Canisius College, Niagara University, Niagara University, New York, Buffalo, New York, USA

    Edwin A. Mirand

  3. Department of Biochemical Pharmacology, School of Pharmacy, State University of New York, Buffalo, New York, USA

    Nathan Back

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© 1969 Springer Science+Business Media New York

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Kellogg, T., Wostmann, B. (1969). The Response of Germfree Rats to Dietary Cholesterol. In: Mirand, E.A., Back, N. (eds) Germ-Free Biology Experimental and Clinical Aspects. Advances in Experimental Medicine and Biology, vol 3. Springer, New York, NY. https://doi.org/10.1007/978-1-4899-6495-3_37

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  • DOI: https://doi.org/10.1007/978-1-4899-6495-3_37

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4899-6234-8

  • Online ISBN: 978-1-4899-6495-3

  • eBook Packages: Springer Book Archive

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