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The Outer Membrane of Acinetobacter: Structure-Function Relationships

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The Biology of Acinetobacter

Part of the book series: Federation of European Microbiological Societies Symposium Series ((FEMS,volume 57))

Abstract

The fine structure of the cell envelope of Acinetobacter has been determined from electron micrographs of thin sections, negatively-stained and freeze-etched preparations of whole cells, and of envelopes treated to remove the various layers (Thornley and Glauert, 1968; Thorne et al., 1973; Sleytr et al., 1974; Thornley and Sleytr, 1974; Aurich et al., 1977). These early studies revealed the presence of an outer membrane typical of Gram-negative bacteria. Furthermore, there are numerous reports of superficial material covering the outer surface of several Acinetobacter strains; such material includes crystalline surface layers of protein (S-layers) (Thornley et al., 1973, 1974), and polysaccharide capsules (Taylor and Juni, 1961; Pines et al., 1983). These additional surface layers are of tremendous importance in determining the surface properties of the cells, and the polysaccharide capsular material has proven to be a potent extracellular emulsifying agent (reviewed by Rosenberg and Kaplan, 1987).

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References

  • Adams, G.A., Oadling, C., Yaguchi, M., and Tornabene, T.G., 1970, The chemical composition of cell wall lipopolysaccharides from Moraxella duplex and Micrococcus calcoaceticus, Can. J. Microbiol., 16:1.

    Article  PubMed  CAS  Google Scholar 

  • Aurich, H., Sorger, H., and Müller, H., 1977, Isolierung und Charakterisierung der Zellgrenzschichten von Acinetobacter calcoaceticus, Zeits. Allg. Mikrobiol., 17:333.

    Article  CAS  Google Scholar 

  • Baker, W.L., 1984, A sensitive procedure for screening microorganisms for the presence of penicillin amidase, Aust. J. Biol. Sci., 37:257.

    PubMed  CAS  Google Scholar 

  • Baumann, P., Doudoroff, M., and Stanier, R.Y., 1968, A study of the Moraxella group. II. Oxidase-negative species (genus Acinetobacter), J. Bacteriol., 95:1520.

    PubMed  CAS  Google Scholar 

  • Benz, R., 1985, Porin from bacterial and mitochondrial outer membranes, CRC Crit. Rev. Biochem., 19:145.

    Article  PubMed  CAS  Google Scholar 

  • Blechschmidt, B., Borneleit, P., Emanouilidis, I., Lehmann, W., and Kleber, H.-P., 1989, Extracellular location of a β-lactamase produced by Acinetobacter calcoaceticus, Appl. Microbiol. Biotech., 32:85.

    Article  CAS  Google Scholar 

  • Borneleit, P., Hermsdorf, T., Claus, R., Walther, P., and Kleber, H.-P., 1988, Effect of hexadecane-induced vesiculation on the outer membrane of Acinetobacter calcoaceticus, J. Gen. Microbiol., 134:1983.

    PubMed  CAS  Google Scholar 

  • Borneleit, P., Blechschmidt, B., Blasig, R., Franke, P., Günther, P., and Kleber, H.-P., 1989a, Preparation of R-type lipopolysaccharide of Acinetobacter calcoaceticus by EDTA-salt extraction, Curr. Microbiol., 19:77.

    Article  CAS  Google Scholar 

  • Borneleit, P., Blechschmidt, B., and Kleber, H.-P., 1989b, Interaction between lipopolysaccharide and outer membrane proteins of Acinetobacter calcoaceticus studied by an affinity electrophoresis system, Electrophoresis, 10:234.

    Article  PubMed  CAS  Google Scholar 

  • Borneleit, P., Binder, H., and Kleber, H.-P., 1990, Outer membrane vesiculation of Acinetobacter calcoaceticus, Acta Biotech., 10:127.

    Article  Google Scholar 

  • Brade, H., and Galanos, C., 1982, Isolation, purification and chemical analysis of the lipopolysaccharide and lipid A of Acinetobacter calcoaceticus NCTC 10305, Eur. J. Biochem., 122:233.

    Article  PubMed  CAS  Google Scholar 

  • Brade, H., and Rietschel, E.T., 1985, Identification of 2-keto-3-deoxy-1,7-dicarboxyheptonic acid as a constituent of the lipopolysaccharide of Acinetobacter calcoaceticus NCTC 10305, Eur. J. Biochem., 153:249.

    Article  PubMed  CAS  Google Scholar 

  • Brade, H., Tacken, A., and Christian, R., 1987, Isolation and identification of a rhamnosyl-rhamnosyl-3-deoxy-D-manno-octulosonic acid trisaccharide from the lipopolysaccharide of Acinetobacter calcoaceticus, Carbohyd. Res., 167:295.

    Article  CAS  Google Scholar 

  • Breuil, C., and Kushner, D.J., 1975a, Lipase and esterase formation by psychrophilic and mesophilic Acinetobacter species, Can. J. Microbiol., 21:423.

    Article  PubMed  CAS  Google Scholar 

  • Breuil, C., and Kushner, D.J., 1975b, Partial purification and characterization of the lipase of a facultatively psychrophilic bacterium (Acinetobacter 016), Can. J. Microbiol., 21:434.

    Article  PubMed  CAS  Google Scholar 

  • Breuil, C., Shindler, D.B., Sijher, J.S., and Kushner, DJ., 1978, Stimulation of lipase production during bacterial growth on alkanes, J. Bacteriol., 133:601.

    PubMed  CAS  Google Scholar 

  • Claus, R., Käppeli, O., and Fiechter, A., 1984, Possible role of extracellular membrane particles in hydrocarbon utilization by Acinetobacter calcoaceticus 69-V, J. Gen. Microbiol., 130:1035.

    CAS  Google Scholar 

  • Claus, R., Fischer, B.E., and Kleber, H.-P., 1985, An esterase as marker enzyme of the outer membrane of Acinetobacter calcoaceticus, J. Basic Microbiol., 25:299.

    Article  CAS  Google Scholar 

  • Fischer, B.E., 1986, Localization of hydrolytic enzymes in Acinetobacter calcoaceticus, J. Basic Microbiol., 26:9.

    Article  PubMed  CAS  Google Scholar 

  • Fischer, B.E., and Kleber, H.-P., 1987, Isolation and characterization of the extracellular lipase of Acinetobacter calcoaceticus 69-V, J. Basic Microbiol., 27:427.

    Article  PubMed  CAS  Google Scholar 

  • Fischer, B.E., Claus, R., and Kleber, H.-P., 1984, Isolation and characterization of the outer membrane of Acinetobacter calcoaceticus, J. Biotech., 1:111.

    Article  CAS  Google Scholar 

  • Fischer, B.E., Koslowski, R., Eichler, W., and Kleber, H.-P., 1987, Biochemical and immunological characterization of lipase during its secretion through cytoplasmic and outer membrane of Acinetobacter calcoaceticus 69 V, J. Biotech., 6:271.

    Article  CAS  Google Scholar 

  • Fricke, B., Jahreis, G., and Sorger, H., 1983, Characterization of membrane-bound endoprotease activities in Acinetobacter, Wiss. Beitr. Martin-Luther-Univ. Halle-Wittenberg, 52:50.

    CAS  Google Scholar 

  • Fricke, B., Jahreis, G., Sorger, H., and Aurich, H., 1986, Cell envelope-bound proteinase activities in Acinetobacter calcoaceticus, Biomed. Biochim. Acta, 45:257.

    PubMed  CAS  Google Scholar 

  • Gustafsson, P., Nordström, K., and Normark, S., 1973, Outer penetration barrier of Escherichia coli K-12: kinetics of the uptake of gentian violet by wild type and envelope mutants, J. Bacteriol., 116:893.

    PubMed  CAS  Google Scholar 

  • Guymon, L.F., and Sparling, P.F., 1975, Altered crystal violet permeability and lytic behaviour in antibiotic-resistant and-sensitive mutants of Neisseria gonorrhoeae, J. Bacteriol., 124:757.

    PubMed  CAS  Google Scholar 

  • Haferburg, D., and Kleber, H.-P., 1982, Extracellular lipase from Acinetobacter calcoaceticus, Acta Biotechnol., 2:337.

    Article  CAS  Google Scholar 

  • Haferburg, D., and Kleber, H.-P., 1983, Regulation of extracellular lipase of an alkane-utilizing Acinetobacter strain, Acta Biotechnol., 3:185.

    Article  CAS  Google Scholar 

  • Hancock, R.E.W., 1984, Alterations in outer membrane permeability, Ann. Rev. Microbiol., 38:234.

    Article  Google Scholar 

  • Hebeler, B.H., Morse, S.A., Wong, W., and Young, F.E., 1978, Evidence for peptidoglycan-associated protein(s) in Neisseria gonorrhoeae, Biochem. Biophys. Res. Comm., 81:1011

    Article  PubMed  CAS  Google Scholar 

  • Henriksen, S.D., 1973, Moraxella, Acinetobacter and Mimeae, Bacteriol. Rev., 37:522.

    PubMed  CAS  Google Scholar 

  • Horisberger, M., and Dentan, E., 1980, Chemical composition and ultrastructure of cellular and extracellular Moraxella glucidolytica lipopolysaccharides, Arch. Microbiol., 128:12.

    Article  PubMed  CAS  Google Scholar 

  • Jahreis, G., and Aurich, H., 1989, Identification and localization of exopeptidase activities bound to membranes of Acinetobacter calcoaceticus, Biomed. Biochim. Acta, 48:517.

    PubMed  CAS  Google Scholar 

  • Joly-Guillou, M.L., Valleé, E., Bergogne-Bérézin, E., and Philippon, A., 1988, Distribution of β-lactamase and phenotype analysis in clinical strains of Acinetobacter calcoaceticus, J. Antimicrob. Chemother., 22:597.

    Article  Google Scholar 

  • Juni, E., 1978, Genetics and physiology of Acinetobacter, Ann. Rev. Microbiol., 32:349.

    Article  CAS  Google Scholar 

  • Juni, E., 1984, Genus III. Acinetobacter Brisou et Prevot 1954, in “Bergey’s Manual of Systematic Bacteriology, vol.1,” p.303, N.R. Greig and J.G. Holt, eds., Williams and Wilkins, Baltimore.

    Google Scholar 

  • Kalogridov-Vassiliadov, D., 1984, Lipolytic activity and heat-resistance of extracellular lipases of some gram-negative bacteria, Milchwissenschaft, 39:601.

    Google Scholar 

  • Käppeli, O., and Finnerty, W.R., 1979, Partition of alkane by an extracellular vesicle derived from hexadecane-grown Acinetobacter, J. Bacteriol., 140:707.

    PubMed  Google Scholar 

  • Katohda, S., Suzuki, F., Katsuki, S., and Sato, T., 1979, Studies on microbial enzyme active in hydrolyzing yeast polysaccharides. Part II. Purification and some properties of endo-beta-1,6-glucanase from Acinetobacter sp., Agric. Biol. Chem., 43:2029.

    Article  CAS  Google Scholar 

  • Kawahara, K., Brade, H., Rietschel, E.T., and Zähringer, U., 1987, Studies on the chemical structure of the core-lipid A region of the lipopolysaccharide of Acinetobacter calcoaceticus NCTC 10305. Detection of a new 2-octulosonic acid interlinking the core oligosaccharide and lipid A component, Eur. J. Biochem., 163:489.

    Article  PubMed  CAS  Google Scholar 

  • Kleber, H.-P., Schopp, W., and Aurich, H., 1973, Verwertung von n-Alkanen durch einen Stamm von Acinetobacter calcoaceticus, Zeits. Allg. Mikrobiol., 13:445.

    Article  CAS  Google Scholar 

  • Kleber, H.-P., Claus, R., and Asperger, O., 1983, Enzymologie der n-Alkanoxidation bei Acinetobacter, Acta Biotech., 3:251.

    Article  CAS  Google Scholar 

  • Lehmann, V., 1971a, Production of phospholipase C in Acinetobacter calcoaceticus, Acta Path. Microbiol. Scand., B79:789.

    Google Scholar 

  • Lehmann, V., 1971b, Phospholipase activity of Acinetobacter calcoaceticus, Acta Path. Microbiol. Scand., B79:372.

    Google Scholar 

  • Lehmann, V., 1972, Properties of purified phospholipase C from Acinetobacter calcoaceticus, Acta Path. Microbiol. Scand., B80:827.

    Google Scholar 

  • Lehmann, V., 1973a, Nature of phospholipase C from Acinetobacter calcoaceticus. Effects of whole red cells and red cell membranes, Acta Path. Microbiol. Scand., B81:419.

    Google Scholar 

  • Lehmann, V., 1973b, Hemolytic activity of various strains of Acinetobacter, Acta Path. Microbiol. Scand., B81:427.

    Google Scholar 

  • Ludewig, M., 1983, An aminopeptidase bound to cell envelopes of Acinetobacter calcoaceticus, Wiss. Beitr. Martin-Luther-Univ. Halle-Wittenberg, 52:47.

    CAS  Google Scholar 

  • Lugtenberg, B., and van Alphen, L., 1983, Molecular architecture and functioning of the outer membrane of Escherichia coli and other gram-negative bacteria, Biochim. Biophys. Acta, 737:51.

    Article  PubMed  CAS  Google Scholar 

  • Lysko, P.G., and Morse, S.A., 1981, Neisseria gonorrhoeae cell envelope: permeability to hydrophobic molecules, J. Bacteriol., 145:946.

    PubMed  CAS  Google Scholar 

  • McDougall, G.J., Fixter, L.M., and Fewson, C.A., 1983, The occurrence of 2-keto-3-deoxy-octonic acid in Acinetobacter calcoaceticus, FEMS Microbiol. Lett., 18:79.

    Article  CAS  Google Scholar 

  • Monboisse, J.-C., Labadie, J., and Gouet, P., 1979a, Attachment of a collagenolytic bacteria to its substrate, Ann. Microbiol., 130A:435.

    CAS  Google Scholar 

  • Monboisse, J.-C., Labadie, J., and Gouet, P., 1979b, Purification and physiochemical properties of collagenase synthesized by Acinetobacter sp. bacteria, Biochimie, 61:1169.

    Article  PubMed  CAS  Google Scholar 

  • Nikaido, H., 1979, Permeability of the outer membrane of bacteria, Ang. Chemie, 18:337.

    Article  CAS  Google Scholar 

  • Nikaido, H., and Vaara, M., 1985, Molecular basis of bacterial outer membrane permeability, Microbiol. Rev., 49:1.

    PubMed  CAS  Google Scholar 

  • Nishimura, Y., Ino, T., and Iizuka, H., 1986, Isolation and characterization of the outer membrane of radiation resistant Acinetobacter sp. FO-1, J. Gen. Appl. Microbiol., 32:177.

    Article  CAS  Google Scholar 

  • Onishi, H., and Hidaka, O., 1978, Purification and properties of amylase produced by a moderately halophilic Acinetobacter species, Can. J. Microbiol., 24:1017.

    Article  PubMed  CAS  Google Scholar 

  • Onishi, H., Hidaka, O., and Nishimura, A., 1979, Halophilic alpha-amylase I and II, Jap. Kokai Tokkyo Koho, 9.

    Google Scholar 

  • Paul, G., Joly-Guillou M.L., Bergogne-Bérézin, E., Nevot, P., and Philippon, A., 1989, Novel carbenicillin-hydrolizing β-lactamase (CARB-5) from Acinetobacter calcoaceticus var. anitratus, FEMS Microbiol. Lett., 59:45.

    CAS  Google Scholar 

  • Pines, O., Bayer, E., and Gutnick, D., 1983, Localization of emulsan-like polymers associated with the cell surface of Acinetobacter calcoaceticus, J. Bacteriol., 154:893.

    PubMed  CAS  Google Scholar 

  • Pugsley, A.P., and Schwartz, M., 1985, Export and secretion of proteins by bacteria, FEMS Microbiol. Rev., 32:3.

    Article  CAS  Google Scholar 

  • Reisfeld, A., Rosenberg, E., and Gutnick, D., 1972, Microbial degradation of crude oil: factors affecting the dispersion in sea water by mixed and pure cultures, J. Appl. Microbiol., 24:363

    CAS  Google Scholar 

  • Rietschel, E.T., Brade, L., Schade, U., Seydel, U., Zahringer, U., Kusumoto, S., and Brade, H., 1987, Bacterial endotoxins: Properties and structure of biologically active domains, in “Surface Structures of Microorganisms and their Interaction with the Mammalian Host,” p.1, E. Schirmer, M.H. Richmond, G. Seibert and U. Schwarz, eds., Hoechst, Ringberg.

    Google Scholar 

  • Rosenberg, E., and Kaplan, N., 1987, Surface-active properties of Acinetobacter exopolysaccharides, in “Bacterial Outer Membranes as Model Systems,” p. 311, M. Inouye, ed., Wiley, New York.

    Google Scholar 

  • Sarubbi, F., Sparling, P.F., Blackman, E., and Lewis, E., 1975, Loss of low-level antibiotic resistance in Neisseria gonorrhoeae due to env mutations, J. Bacteriol., 124:750.

    PubMed  CAS  Google Scholar 

  • Scott, C., Makula, R.A., and Finnerty, W.R., 1976, Isolation and characterization of membranes from a hydrocarbon-oxidizing Acinetobacter sp., J. Bacteriol., 127:469.

    PubMed  CAS  Google Scholar 

  • Singer, M.E., Tyler, S.M., and Finnerty, W.R., 1985, Growth of Acinetobacter sp. strain HO1-N on hexadecanol: physiological and ultrastructural characteristics, J. Bacteriol., 162:162.

    PubMed  CAS  Google Scholar 

  • Sleytr, U.B., Thornley, M.J., and Glauert, A.M., 1974, Location of the fracture faces within the cell envelope of Acinetobacter species strain MJT/F5/5, J. Bacteriol., 118:693.

    PubMed  CAS  Google Scholar 

  • Stewart, J.E., Kallio, R.E., Stevenson, D.P., Jones, A.C., and Schissler, D.O., 1959, Bacterial hydrocarbon oxidation. I. Oxidation of n-hexadecane by a gram-negative coccus, J. Bacteriol., 78:441

    PubMed  CAS  Google Scholar 

  • Taylor, W.H., and Juni, E., 1961, Pathways for biosynthesis of bacterial capsular polysaccharide. I. Characterization of the organism and polysaccharide, J. Bacteriol., 81:688.

    PubMed  CAS  Google Scholar 

  • Thorne, K.J.I., Thornley, M.I., and Glauert, A.M., 1973, Chemical analysis of the outer membrane and other layers of the cell envelope of Acinetobacter sp., J. Bacteriol., 116:410.

    PubMed  CAS  Google Scholar 

  • Thorne, K.J.I., Oliver, R.C., and Heath, M.F., 1976, Phospholipase A2 activity of the regularly arranged surface protein of Acinetobacter sp. 199A, Biochim. Biophys. Acta, 450:335.

    Article  PubMed  CAS  Google Scholar 

  • Thornley, M.J., and Glauert, A.M., 1968, Fine structure and radiation resistance in Acinetobacter: studies on a resistant strain, J. Cell Sci., 3:273.

    PubMed  CAS  Google Scholar 

  • Thornley, M.J., and Sleytr, U.B., 1974, Freeze-etching of the outer membranes of Pseudomonas and Acinetobacter, Arch. Microbiol., 100:409.

    Article  PubMed  CAS  Google Scholar 

  • Thornley, M.J., Glauert, A.M., and Sleytr, U.B., 1973, Isolation of outer membranes with an ordered array of subunits from Acinetobacter, J. Bacteriol., 115:1294.

    Google Scholar 

  • Thornley, M.J., Thorne, K.J.I., and Glauert, A.M., 1974, Detachment and chemical characterization of the regularly arranged subunits from the surface of Acinetobacter, J. Bacteriol., 118:654.

    PubMed  CAS  Google Scholar 

  • Torregrossa, R.E., Makula, R.A., and Finnerty, W.R., 1977, Outer membrane phospholipase A from Acinetobacter sp. HO1-N, J. Bacteriol., 131:493.

    PubMed  CAS  Google Scholar 

  • Torregrossa, R.E., Makula, R. A., and Finnerty, W.R., 1978, Hydrolysis of phosphatidylethanolamine and phosphatidylglycerol by outer membrane phospholipase A from Acinetobacter sp. HO1-N, J. Bacteriol., 136:803.

    PubMed  CAS  Google Scholar 

  • Zaikina, N.A., and Robakidze, T.N., 1976, Phospholipase C of fungi and staphylococci, Mikrobiologiya, 45:466.

    CAS  Google Scholar 

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  1. Sektion Biowissenschaften Bereich Biochemie, Karl-Marx-Universität Leipzig, Talstrasse 33, 7010, Leipzig, Germany

    P. Borneleit & H.-P. Kleber

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  1. P. Borneleit
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  2. H.-P. Kleber
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Editors and Affiliations

  1. Department of Microbiology and PHLS Laboratory, University Hospital, Nottingham, UK

    K. J. Towner

  2. Département de Microbiologie, CHU Bichat, Paris, France

    E. Bergogne-Bérézin

  3. Department of Biochemistry, University of Glasgow, Glasgow, UK

    C. A. Fewson

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Borneleit, P., Kleber, HP. (1991). The Outer Membrane of Acinetobacter: Structure-Function Relationships. In: Towner, K.J., Bergogne-Bérézin, E., Fewson, C.A. (eds) The Biology of Acinetobacter . Federation of European Microbiological Societies Symposium Series, vol 57. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3553-3_18

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