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Cultured Intestinal Epithelial Cell Models

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Models for Assessing Drug Absorption and Metabolism

Part of the book series: Pharmaceutical Biotechnology ((PBIO,volume 8))

Abstract

In vitro systems such as brush border membrane vesicles (BBMV), basolateral membrane vesicles (BLMV), perfused intestinal loops, stripped intestinal mucosa, and isolated enterocytes have been used to study mucosal drug absorption. Isolated enterocytes should permit the determination of transmembrane transport in the presence of cellular metabolism. However, upon isolation enterocytes lose their polarity and show limited viability (Hartmann et al., 1982). Attempts to develop an in vitro system derived from cultured intestinal epithelial cells have found this goal to be extremely difficult. Although intestinal enterocytes have been cultured in suspension, they undergo dedifferentiation when cultured as monolayers (Raul et al., 1978).

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Author information

Authors and Affiliations

  1. Drug Metabolism and Pharmacokinetics (NW12), Rhone-Poulenc Rorer, Collegeville, Pennsylvania, 19427-0107, USA

    Ismael J. Hidalgo

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  1. Ismael J. Hidalgo
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Editor information

Editors and Affiliations

  1. The University of Kansas, Lawrence, Kansas, USA

    Ronald T. Borchardt

  2. SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania, USA

    Philip L. Smith

  3. Tacora Corporation, Seattle, Washington, USA

    Glynn Wilson

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© 1996 Springer Science+Business Media New York

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Hidalgo, I.J. (1996). Cultured Intestinal Epithelial Cell Models. In: Borchardt, R.T., Smith, P.L., Wilson, G. (eds) Models for Assessing Drug Absorption and Metabolism. Pharmaceutical Biotechnology, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1863-5_3

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  • DOI: https://doi.org/10.1007/978-1-4899-1863-5_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1865-9

  • Online ISBN: 978-1-4899-1863-5

  • eBook Packages: Springer Book Archive

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