Abstract
Eukaryotic cells contain an extensive filamentous network referred to as the cytoskeleton, and it is currently believed that all animal viruses may use the cytoplasmic or nuclear skeletal matrix of cells for at least part of their replication cycle (Luftig, 1982; Penman, 1985). Analysis of cells infected with double-stranded RNA-containing reoviruses has done much to stimulate interest in the role played by the cytoskeleton in viral replication (Dales, 1963; Spendlove et al., 1963; Dales et al., 1965; Sharpe et al., 1982). The purpose of this chapter is to describe the results of experiments done in our laboratory to analyze cytoskeleton involvement in the replication of another double-stranded RNA virus, bluetongue virus (BTV), a member of the orbivirus genus in the Reoviridae family.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Babiss, L. E., Luftig, R. B., Weatherbee, J. A., Weihing, R. R., Ray, U. R., and Fields, B. N., 1979, Reovirus serotypes 1 and 3 differ in their in vitro association with microtubules, J. Virol. 30:863–874.
Bedows, E., Rao, K. M. K., and Welsh, M. J., 1983, Fate of microfilaments in Vero cells infected with measles virus and herpes simplex virus type 1, Mol. Cell. Biol. 3:712–719.
Ben-Ze’ev, A., 1984, Inhibition of vimentin synthesis and disruption of intermediate filaments in simian virus 40-infected monkey kidney cells, Mol. Cell Biol. 4:1880–1889.
Ben-Ze’ev, A., Abulafia, R., and Bratosin, S., 1983, Herpes simplex virus and protein transport are associated with the cytoskeletal framework and the nuclear matrix in infected BSC-1 cells, Virology 129:501–507.
Blose, S. H., and Chacko, S., 1976, Rings of intermediate (100A) filemant bundles in the perinuclear region of vascular endothelial cells. Their mobilization by colcemid and mitosis, J. Cell Biol. 70:459–466.
Bonn, W., Rutter, G., Hohenberg, H., Mannweiler, K., and Nobis, P., 1986, Involvement of actin filaments in budding of measles virus: Studies on cytoskeletons of infected cells, Virology 149:91–106.
Boschek, C. B., Jockusch, B. M., Friis, R. R., Back, R., Grundmann, E., and Bauer, H., 1981, Early changes in the distribution and organization of microfilament proteins during cell transformation, Cell 24:175–184.
Bowne, J. G., and Jochim, M. M., 1967, Cytopathologic changes and development of inclusion bodies in cultured cells infected with bluetongue virus, Am. J. Vet. Res. 28:1091–1105.
Bowne, J. G., and Ritchie, A. E., 1970, Some morphological features of bluetongue virus, Virology 40:903–911.
Brown, S., Levinson, W., and Spudich, J. A., 1976, Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction, J. Supramol. Struct. 5:119–130.
Chatterjee, P. K., Cervera, M. M., and Penman, S., 1984, Formation of vesicular stomatitis virus nucleocapsid from cytoskeletal framework-bound N protein: Possible model for structure assembly, Mol. Cell. Biol. 4:2231–2234.
Cromack, A. S., Blue, J. L., and Gratzek, J. B., 1971, A quantitative ultrastructural study of the development of bluetongue virus in Madin-Darby bovine kidney cells, J. Gen. Virol. 13:229–244.
Dales, S., 1963, Association between the spindle apparatus and reovirus, Proc. Natl. Acad. Sci. USA 50:268–275.
Dales, S., and Chardonnet, Y., 1973, Early events in the interaction of adenoviruses with HeLa cells. IV. Association with microtubules and the nuclear pore complex during vectorial movement of the inoculum, Virology 56:465–483.
Dales, S., Gomatos, P. J., and Hsu, K. C., 1965, The uptake and development of reovirus in strain L cells followed with labeled viral ribonucleic acid and ferritin-antibody conjugates, Virology 25:193–211.
Drochmans, P., Freudenstein, C., Wanson, J. C., Laurent, L., Keenan, T. W., Stadler, J., LeLoup, R., and Franke, W. W., 1978, Structure and biochemical composition of desmosomes and tonofilaments isolated from calf muzzle epidermis, J. Cell Biol. 79:427–443.
Eaton, B. T., Hyatt, A. D., and White, J. R., 1987, Association of bluetongue virus with the cytoskeleton, Virology 157:107–116.
Eaton, B. T., Hyatt, A. D., and White, J. R., 1988, Localization of the nonstructural protein NS1 in bluetongue virus infected cells and its presence in virus particles, Virology 163:527–537.
Edbauer, C. A., and Naso, R. B., 1983, Cytoskeleton-associated Pr65sag and retrovirus assembly, Virology 130:415–426.
Franke, W. W., Schmid, E., Weber, K., and Osborn, M, 1979, HeLa cells contain intermediatesized filaments of the prekeratin type, Exp. Cell. Res. 118:95–109.
Goldman, R. D., 1971, The role of three cytoplasmic fibres in BHK-21 cell motility. 1. Microtubules and the effects of colchicine, J. Cell Biol. 51:752–762.
Goldman, R. D., Chang, C., and Williams, J. F., 1975, Properties and behaviour of hamster embryo cells transformed by human adenovirus type 5, Cold Spring Harbor Symp. Quant. Biol. 39:601–614.
Gorman, B. M., and Taylor, J., 1985, Orbiviruses, in: Virology (B. N. Fields, ed.) pp. 907–925, Raven, New York.
Gould, A. R., Hyatt, A. D., and Eaton, B. T., 1988, Morphogenesis of a bluetongue virus variant with an amino acid alteration at a neutralization site in the outer coat protein, VP2, Virology 165:23–32.
Grubman, M. J., Appleton, J. A., and Letchworth, G. L., 1983, Identification of bluetongue virus type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity, Virology 131:355–366.
Hartwig, J. H., Niederman, R., and Lind, S. E., 1985, Cortical actin structures and their relationship to mammalian cell movements, Subcell. Biochem. 11:1–49.
Hayat, M. A., 1986, Micron Microsc. Acta 17:115–135.
Hiller, G., Weber, K., Schneider, L., Parajsz, C., and Jungwirth, C., 1979, Interaction of assembled progeny pox viruses with the cellular cytoskeleton, Virology 98:142–153.
Howard, J. M., Eckert, B. S., and Bourguignon, L. Y. W., 1983, Comparison of cytoskeletal organization in canine distemper virus-infected and uninfected cells, J. Gen. Virol. 64:2379–2385.
Hronis, T. S., Steinberg, M. L., Defendi, V., and Sun, T. T., 1984, Simple epithelial nature of some simian virus-40-transformed human epidermal keratinocytes, Cancer Res. 44:5797–5804.
Huismans, H., and Cloete, M., 1987, A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specified RNA, Virology 158:373–380.
Huismans, H., and Els, H. J., 1979, Characterization of the tubules associated with the replication of three different orbiviruses, Virology 92:397–406.
Huismans, H., and Verwoerd, D. W., 1973, Control of transcription during the expression of the bluetongue virus genome, Virology 52:81–88.
Huismans, H., Van Der Walt, N. T., Cloete, M., and Erasmus, B. J., 1987a, Isolation of a capsid protein of bluetongue virus that induces a protective immune réponse in sheep, Virology 157:172–179.
Huismans, H., Van Dijk, A. A., and Bauskin, A. R., 1987b, In vitro phosphorylation and purification of a non structural protein of bluetongue virus with affinity for single-stranded RNA, J. Virol. 61:3589–3595.
Huismans, H., Van Dijk, A. A., and Els, H. J., 1987c, Uncoating of parental bluetongue virus to core and subcore particles in infected L cells, Virology 157:180–188.
Hyatt, A. D., and Eaton, B. T., 1988, Ultrastructural distribution of the major capsid proteins within bluetongue virus and infected cells, J. Gen. Virol. 69:805–815.
Hyatt, A. D., Eaton, B. T., and Lunt, R., 1987, The grid-cell-culture technique: The direct examination of virus-infected cells and progeny viruses, J. Microsc. 145:97–106.
Jackson, P., and Bellett, A. J. D., 1985, Reduced microfilament organization in adenovirus type 5-infected rat embryo cells: A function of early region la, J. Virol. 55:644–650.
Jones, N. L., Lewis, J. C., and Kilpatrick, B. A., 1986, Cytoskeletal disruption during human cytomegalovirus infection of human lung fibroblasts, Eur. J. Cell. Biol. 41:304–312.
Knipe, D. M., and Spang, A. E., 1982, Definition of a series of stages in the association of two herpesviral proteins with the cell nucleus, J. Virol. 43:314–324.
Lecatsas, G., 1968, Electron microscopic study of the formation of bluetongue virus, Onderstepoort J. Vet. Res. 35:139–150.
Lenk, R., Ransom, L., Kaufmann, V., and Penman, S., 1977, A cytoskeletal structure with associated polyribosomes obtained from HeLa cells, Cell 10:67–78.
Luftig, R. B., 1982, Does the cytoskeleton play a significant role in animal virus replication, J. Theor. Biol. 99:173–191.
Luftig, R. B., and Weihing, R. R., 1975, Adenovirus binds to rat brain microtubules in vitro, J. Virol. 16:696–706.
Mertens, P. P. C., Brown, F., and Sangar, D. V., 1984, Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode, Virology 135:207–217.
Miles, B.D., Luftig, R. B., Weatherbee, J. A., Weihing, R. R., and Weber, J., 1980, Quantitation of the interaction between adenovirus types 2 and 5 and microtubules inside infected cells, Virology 105:265–269.
Moll, R., Franke, W. W., Schiller, D. L., Greiger, B., and Krepier, R., 1982, The catalog of human cytokerations: Patterns of expression in normal epithelial, tumors and cultured cells, Cell 31:11–24.
Mora, M., Partin, K., Bhatia, M., Partin, J., and Carter, C., 1987, Association of reovirus proteins with the structural matrix of infected cells, Virology 159:265–277.
Murti, K. G., Goorha, R., and Chen, M., 1985, Interaction of frog virus 3 with the cytoskeleton, Curr. Top. Microbiol. Immunol. 116:107–131.
Murti, K. G., Chen, M., and Goorha, R., 1985, Interaction of frog virus 3 with the cytomatrix. III. Role of microfilaments in virus release, Virology 142:317–325.
Musalem, C., and Espejo, R. T., 1985, Release of progeny virus from cells infected with simian rotavirus SAH, J. Gen. Virol. 66:2715–2724.
Nielsen, L. N., Forchhammer, J., Dabelsteen, E., Jepsen, A., Teglbjaerg, C. S., and Norrild, B., 1987, Herpes simplex virus-induced changes of the keratin type intermediate filament in rat epithelial cells, J. Gen. Virol. 68:737–748.
Norrild, B., Lehto, V.-P., and Virtanen, L, 1986, Organization of cytoskeleton elements during herpes simplex virus type 1 infection of human fibroblasts: An immunofluorescence study, J. Gen. Virol. 67:97–105.
Pasquale, E. B., Maher, P. A., and Singer, S. J., 1986, Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous Sarcoma Virus, Proc. Natl. Acad. Sci. USA 83:5507–5511.
Penman, S., 1985, Virus metabolism and cellular architecture, in: Virology (B. N. Fields, ed.), pp. 169–182, Raven, New York.
Penman, S., Capco, D., Fey, E., Chatterjee, P., Reiter, T., Ermish, S., and Wan, K., 1983, The three dimensional structural networks of cytoplasm and nucleus: Function in cells and tissue, in J. R. Mclntosh and B. H. Satir (eds.), Modern Cell Biology, Vol. 2, pp. 385–415, Liss, New York.
Pfeiffer, G., Willutzki, D., Weder, D., Becker, B., and Radsak, K., 1983, Microtubular reaction in human fibroblasts infected with cytomegalovirus, Arch. Virol. 76:153–159.
Pollack, R., Osborn, M., and Weber, K., 1975, Patterns of organization of actin and myosin in normal and transformed cultured cells, Proc. Natl. Acad. Sci. USA 72:994–998.
Rose, D. S. C., Shriver, K., Latchman, D. S., and LaThangue, N. B., 1986, A filamentous distribution for the herpes simplex virus type 2-encoded major DNA-binding protein, J. Gen. Virol. 67:1315–1325.
Rutter, G., and Mannweiler, K., 1977, Alterations of actin-containing structures in BHK21 cells infected with Newcastle Disease virus and vesicular stomatitis virus, J. Gen. Virol. 37:233–242.
Sefton, B. M., Hunter, T., Ball, E. H., and Singer, S. J., 1981, Vinculin: A cytoskeletal target of the transforming protein of Rous Sarcoma virus, Cell 24:165–174.
Sharpe, A. H., Chen, L. B., and Fields, B. N., 1982, The interaction of mammalian reoviruses with the cytoskeleton of monkey kidney CV-1 cells, Virology 120:399–411.
Spendlove, R. S., Lennette, E. H., Knight, C. O., and Chin, J. H., 1963, Development of viral antigen and infectious virus in HeLa cells infected with reovirus, J. Immunol. 90:548–553.
Verwoerd, D. W., Louw, H., and Oellermann, R. A., 1970, Characterization of bluetongue virus ribonucleic acid, J. Virol. 5:1–7.
Verwoerd, D. W., Els, H. J., De Villiers, E. M., and Huismans, H., 1972, Structure of the bluetongue virus capsid, J. Virol. 10:783–794.
Weatherbee, J. A., Luftig, R. B., and Weihing, R. R., 1977, Binding of adenovirus to microtubules. II. Depletion of high-molecular-weight microtubule-associated protein content reduces specificity of in vitro binding, J. Virol. 21:732–742.
Weed, H. G., Krochmalnic, G., and Penman, S., 1985, Poliovirus metabolism and the cytoskeletal framework: Detergent extraction and resinless section electron microscopy, J. Virol. 56:549–557.
White, J. R., Breschkin, A. M., and Della-Porta, A. J., 1985, Immunochemical analysis of Australian bluetongue virus serotypes using monoclonal antibodies, in: Bluetongue and Related Orbiviruses (T. L. Barber and M. M. Jochim, eds.), pp. 397–405, Liss, New York.
Wiche, G., 1985, High-molecular-weight microtubule associated proteins (MAPS): A ubiquitous family of cytoskeletal connecting links, Trends Biochem. Sci. 10:67–70.
Yamauchi, M., Nishiyama, Y., Fujioka, H., Isomura, S., and Maeno, K., 1985, On the intracellular transport and the nuclear association of human cytomegalovirus structural proteins, J. Gen. Virol. 66:675–684.
Yen, S. H., and Fields, K. L., 1981, Antibodies to neurofilament, glial filament and fibroblast intermediate filament proteins bind to different cell types of the nervous system, J. Cell Biol. 88:115–126.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Eaton, B.T., Hyatt, A.D. (1989). Association of Bluetongue Virus with the Cytoskeleton. In: Harris, J.R. (eds) Virally Infected Cells. Subcellular Biochemistry, vol 15. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1675-4_8
Download citation
DOI: https://doi.org/10.1007/978-1-4899-1675-4_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-1677-8
Online ISBN: 978-1-4899-1675-4
eBook Packages: Springer Book Archive