Abstract
Muscle development involves a complex series of events. Mesodermal progenitor cells commit to become myoblasts, myoblasts fuse and, accompanied by cell cycle arrest, differentiate to myotubes. As a cell culture paradigm to a portion of the differentiation process we have chosen to study murine C2C12 cells. C2C12 myoblasts remain undifferentiated and proliferative when grown in the presence of serum due to cell cycle regulated gene products (1). Myoblasts produce virtually no acetylcholinesterase (AChE). Withdrawal of serum stops cell division and induces fusion of myoblasts into multinucleate myotubes which produce both cell associated and exported AChE.
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Camp, S., Taylor, P. (1998). Intronic Elements Appear Essential for the Differentiation-Specific Expression of Acetylcholinesterase in C2C12 Myotubes. In: Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. (eds) Structure and Function of Cholinesterases and Related Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1540-5_7
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DOI: https://doi.org/10.1007/978-1-4899-1540-5_7
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