Summary
By an in vitro chemotaxis assay, we have shown that rat brain macrophages in culture release a soluble factor which stimulates the migration of bone marrow-derived macrophages. This activity was significantly decreased by an immune serum directed against the rat monocyte chemoattractant protein-1 (chemokine MCP-1). By Northern blot analysis, we have shown that MCP-1 is indeed synthetized by brain macrophages in culture, or by astrocytes after in vitro activation. We then evidenced an in vivo production of MCP-1 in the adult rat brain following injury induced by a local injection of kainic acid. This synthesis is localized in both astrocytes and brain macrophages by immunocytochemistry. Altogether, these results suggest that microglial and astroglial secretion of chemokines could contribute to the mechanism(s) leading to the infiltration of the central nervous system by blood-derived monocytes as observed in several pathologies.
Résumé
Dans une étude réalisée chez le rat, nous avons montré que le milieu conditionné de macrophages cérébraux en culture contient une activité chimiotactique s’exerçant sur des macrophages provenant d’une culture de moelle osseuse. Cette activité est fortement inhibée par un anticorps dirigé contre la chemokine MCP-1 de rat. Par ailleurs, l’expression du messager de cette protéine a été detectée par Northern blot, dans des macrophages cérébraux en culture et dans des astrocytes stimulés par le LPS. Par la suite, nous avons montré que l’expression de MCP-1 était induite in vivo lors d’une lésion cérébrale produite par injection d’acide kainic dans le striatum. La synthèse de la protéine a été détectée dans les astrocytes et dans les macrophages cérébraux, suggèrant l’intervention de ces deux types cellulaires dans le (s) mécanisme (s) conduisant à l’infiltration du système nerveux central par des monocytes sanguins observée au cours de certaines pathologies.
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Calvo, CF., Mallat, M. (1996). Expression of Macrophage Chemotactic Protein-1 in Rat Glial Cells. In: Couraud, PO., Scherman, D. (eds) Biology and Physiology of the Blood-Brain Barrier. Advances in Behavioral Biology, vol 46. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9489-2_44
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DOI: https://doi.org/10.1007/978-1-4757-9489-2_44
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