Abstract
The other chapters in this book give the reader an in-depth description of almost every important aspect of biological confocal microscopy that we could think of. This chapter is to provide the novice user of this instrument with a basic understanding of the practical information needed to use it effectively. Because the computer interfaces of the various commercial instruments vary greatly, this chapter will stress the important features of microscopical optics and the basics of sampling that are common to all instruments. The underlying agenda of these suggestions is based on two principles:
-
Don’t waste photons.
-
Get all of the optical performance that you have paid for.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bradbury, S., 1984, An Introduction to the Optical Microscope, Microscope Handbooks, 1, Royal Microscopical Society and Oxford University Press, London.
Inoué, S., 1986, Video Microscopy, Plenum Press, New York.
Mcintosh, J.R., Coue, M., and Vigers, G.P.A., 1990, Fluorescent microtubules are light sensitive, in: Proceedings of the International Conference on Video Microscopy, Chapel Hill, NC (B. Herman and K. Jacobson, eds.), Wiley-Liss, New York, pp. 173–181.
Pawley, J.B., and Centoze, V.E., 1994, Practical laser scanning light microscopy. In: Cell Biology: A Laboratory Handbook, Vol. 2 (J. Celiz, ed.), Academic Press, New York, pp. 44–64.
Pawley, J.B., Amos, W.B., Dixon, A., and Brelje, T.C., 1993, Simultaneous, non-interfering, collection of optimal fluorescent and backscattered light signals on the MRC-500/600, Proc. Microsc. Soc. Am. 51:156–157.
Rose, A., 1948, Television pickup tubes and the problem of vision, Adv. Electron. 1:131–166.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Centonze, V., Pawley, J. (1995). Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_36
Download citation
DOI: https://doi.org/10.1007/978-1-4757-5348-6_36
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-5350-9
Online ISBN: 978-1-4757-5348-6
eBook Packages: Springer Book Archive