Skip to main content
SpringerLink
Log in

Neoplastic Human Glia Cells in Culture

  • Chapter
Human Tumor Cells in Vitro

Abstract

The panorama of human malignant tumors is by far the best described in nature. Over 100 different histogenetic varieties are known, and their biological traits have been abundantly documented. Their in vitro characterization lags far behind, however, mainly because of the considerable technical difficulties involved in establishing cultures from human neoplastic tissue. Primary explants usually yield only necrobiotic neoplastic cells which rapidly die out. Such cultures have found a limited use in connection with certain morphological problems, but have in the main been disappointing. Permanent lines of proven neoplastic origin which would be of much greater general value are still few in number. It would be of immense importance if a method could be invented by which all types of neoplasia could be reproducibly grown in the laboratory in long-term culture.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abercrombie, M., Heaysman, J. E. M., and Pegrum, S. M., 1970a, The locomotion of fibroblasts in culture. I. Movements of the leading edge, Exp. Cell Res. 59: 393.

    Article  PubMed  CAS  Google Scholar 

  • Abercrombie, M., Heaysman, J. E. M., and Pegrum, S. M., 19706, The locomotion of fibroblasts in culture. II. “Ruffling,” Exp. Cell Res. 60: 437.

    Google Scholar 

  • Agren, G., and Ronquist, G., 1969, Formation of extracellular adenosine triphosphate by tumour cells, Acta Physiol. Scand. 75: 124.

    Article  PubMed  CAS  Google Scholar 

  • Agren, G., Nilsson, K., Pontén, J., Ronquist, G., and Westermark, B., 1971a, Formation of extracellular adenosine triphosphate by normal and neoplastic human cells in culture, J. Cell. Physiol. 77: 331.

    Article  PubMed  CAS  Google Scholar 

  • Agren, G., Pontén, J., Ronquist, G., and Westermark, B., 1971b, Demonstration of an ATPase at the cell surface of intact normal and neoplastic human cells in culture, J. Cell. Physiol. 78: 171.

    Article  PubMed  CAS  Google Scholar 

  • Beckman, G., Beckman, L., Pontén, J., and Westermark, B., 1971, G-6-PD and PGM phenocytes of 16 continuous human tumor cell lines, Hum. Hered. 21: 238.

    Article  PubMed  CAS  Google Scholar 

  • Brunk, U., Ericsson, J., Pontén, J., and Westermark, B., 1971, Specialization of cell surfaces in contact-inhibited human glia-like cells in vitro, Exp. Cell Res. 67: 407.

    Article  PubMed  CAS  Google Scholar 

  • Dulbecco, R., 1970, Topoinhibition and serum requirement of transformed and untransformed cells, Nature (Lond.) 227: 802.

    Article  CAS  Google Scholar 

  • Eagle, H., 1959, Amino acid metabolism in mammalian cell cultures, Science 130: 432.

    Article  PubMed  CAS  Google Scholar 

  • Espmark, J. A., Ahlqvist-Roth, L., Chapple, P. J., and Cartwright, L. N., 1973, Alloantigenic characterization of permanent human cell lines, presented at the ASM Meeting, Miami Beach, May 6—l1, Paper M325.

    Google Scholar 

  • Forsby, N., Brunk, U., Ericsson, J., Pontén, J., and Westermark, B., 1972, Ultrastructural features of in vitro-cultivated malignant gliomas, Acta Pathol. Microbiol. Scand. Sect. A 80: 430.

    Google Scholar 

  • Gartler, S. M., 1967, Genetic markers as tracers in cell culture, in: Decennial Review Conference on Cell Tissue and Organ Culture, Natl. Cancer Inst. Monogr. 26: 167.

    Google Scholar 

  • Glimelius, B., Westermark, B., and Pontén, J., 1974, Agglutination of normal and neoplastic human cells by concanavalin A and ricinus communis agglutinin, Int. J. Cancer 14: 314.

    Article  PubMed  CAS  Google Scholar 

  • Ham, R. G., 1963, An improved nutrient solution for diploid Chinese hamster and human cell lines, Exp. Cell Res. 29: 515.

    Article  PubMed  CAS  Google Scholar 

  • Hayflick, L., 1965, The limited in vitro lifetime of human diploid cell strains, Exp. Cell Res. 37: 614.

    Article  PubMed  CAS  Google Scholar 

  • Hayflick, L., and Moorhead, P., 1961, The serial cultivation of human diploid cell strains, Exp. Cell Res. 25: 585.

    Article  PubMed  CAS  Google Scholar 

  • Kersting, G., 1961, Die Gewebszuchtung menschlicher Hirngeschwulste, Springer, Berlin, Gottingen, Heidelberg.

    Google Scholar 

  • Kersting, G., 1964, Tissue culture and the classification of brain tumours, in: Classification of Brain Tumours (K. J. Zülch, and A. L. Woolf, eds.), Acta Neurochir. Suppl. 10: 68.

    Chapter  Google Scholar 

  • Lipton, A., Klinger, I., Paul, D., and Holley, R. W., 1971, Migration of mouse 3T3 fibroblasts in response to a serum factor, Proc. Natl. Acad. Sci. 68: 2799.

    Article  PubMed  CAS  Google Scholar 

  • Lumsden, C. E., 1963, Tissue culture in relation to tumours of the nervous system, in: Pathology of Tumours of the Nervous System, 2nd ed. Lumsden, C. E., eds.), p. 281, Edward Arnold (London).

    Google Scholar 

  • Macintyre, E. H., and Pontén, J., 1967, Interaction between normal and transformed bovine fibroblasts in culture. I. Cells transformed by Rous sarcoma virus, J. Cell Sci. 2: 309.

    PubMed  CAS  Google Scholar 

  • Macintyre, E. H., Grimes, R. A., and Vatter, A. E., 1969, Cytology and growth characteristics of human tumour astrocytes transformed by Rous sarcoma virus, J. Cell Sci. 5: 583.

    PubMed  CAS  Google Scholar 

  • Manuelidis, E. E., 1965, Long-term lines of tissue cultures of intracranial tumors, J. Neurosurg. 22: 368.

    Article  PubMed  CAS  Google Scholar 

  • Manuelidis, E. E., 1969, Experiments with tissue culture and heterologous transplantation of tumors, Ann. N.Y. Acad. Sci. 159: 409.

    Article  Google Scholar 

  • Maunoury, R., Vedrenne, C., Arnoult, J., Constans, J. P., and Febvre, H., 1972, Culture in vitro de tissu glial normal et néoplastique. Croissance, cytologie, ultrastructure, Neuro-chirurgie, Paris 18: 8.

    Google Scholar 

  • McAllister, R. M., Melnyk, J., Finklestein, J. Z., Adams, E. C., Jr., and Gardner, M. B., 1969, Cultivation in vitro of cells derived from a human rhabdomyosarcoma, Cancer 24: 520.

    Article  PubMed  CAS  Google Scholar 

  • McAllister, R. M., Nelson-Rees, W. A., Johnson, E. Y., Rongey, R. W., and Gardner, M. B., 1971, Disseminated rhabdomyosarcomas formed in kittens by cultured human rhabdomyosarcoma cells, J. Natl. Cancer Inst. 47: 603.

    PubMed  CAS  Google Scholar 

  • Moore, G. E., and Koike, K., 1964, Growth of human tumor cells in vitro and in vivo, Cancer 17: 11.

    Article  PubMed  CAS  Google Scholar 

  • Moore, G. E., Gerner, R. E., and Franklin, H. A., 1967, Culture of normal human leukocytes, DAMA 199: 519.

    Article  CAS  Google Scholar 

  • Nakai, J., 1963, Morphology of Neuroglia, Charles C Thomas, Springfield, I.

    Google Scholar 

  • Nelson-Rees, W. A., Klement, V., Peterson, W. D., Jr., and Weawer, J. F., 1973, Comparative study of two RD 114 virus-indicator cell lines, KC and KB, J. Natl. Cancer Inst., submitted.

    Google Scholar 

  • Nicolson, G. L., 1973, Temperature-dependent mobility of concanavalin sites on tumour cell surfaces, Nature (Land.) 243: 218.

    Article  CAS  Google Scholar 

  • Nilsson, K., Pontén, J., and Philipson, L., 1968, Development of immunocytes and immunoglobulin production in long term cultures from normal and malignant human lymph nodes, Int. J. Cancer 3: 183.

    Article  PubMed  CAS  Google Scholar 

  • Nilsson, K., Bennich, H., Johansson, S. G. O., and Pontén, J., 1970, Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgC) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477.

    PubMed  CAS  Google Scholar 

  • Oettgen, H. F., Tadao, A., Old, L. J., Boyse, E. A., de Harven, E., and Mills, G. M., 1968, Suspension culture of pigment-producing cell line derived from a human malignant melanoma, J. Natl. Cancer Inst. 41: 827.

    CAS  Google Scholar 

  • Peterson, W. D., Jr., Stulberg, C. S., Swanborg, N. K., and Robinson, A. R., 1968, Glucose-6phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose—agar gel and cellulose acetate zymograms, Proc. Soc. Exp. Biol. Med. 128: 772.

    Article  PubMed  CAS  Google Scholar 

  • Pontén, J., 1971, Spontaneous and virus induced transformation in cell culture, in: Virology Monographs ( S. Gard, C. Hallauer, and K. F. Meyer, eds.), Wien, New York.

    Google Scholar 

  • Pontén, J., 1973, Transformation of normal human glia cells by a chemical carcinogen. XVII Scandinavian Congress of Pathology and Microbiology, Stockholm. June 12–14, Abstract.

    Google Scholar 

  • Pontén, J., and Macintyre, E. H., 1968, Long term culture of normal and neoplastic human glia, Acta Pathol. Microbiol. Scand. 74: 465.

    Article  PubMed  Google Scholar 

  • Pontén, J., and Saksela, E., 1967, Two established in vitro cell lines from human mesenchymal tumours, Int. J. Cancer 2: 434.

    Article  PubMed  Google Scholar 

  • Pontén, J., Jensen, F., and Koprowski, H., 1963, Morphological and virological investigation of human tissue cultures transformed with SV40, J. Cell. Comp. Physiol. 61: 145.

    Article  PubMed  Google Scholar 

  • Pontén, J., Westermark, B., and Hugosson, H., 1969, Regulation of proliferation and movement of human glia-like cells in ‘culture, Exp. Cell Res. 58: 343.

    Article  Google Scholar 

  • Sharon, N., and Lis, H., 1972, Lectins: Cell-agglutinating and sugar-specific proteins, Science 177: 949.

    Article  PubMed  CAS  Google Scholar 

  • Swim, H. E., and Parker, R. F., 1957, Culture characteristics of human fibroblasts propagated serially, Am. J. Hyg. 66: 235.

    PubMed  CAS  Google Scholar 

  • Temin, H. M., 1971, Stimulation by serum of multiplication of stationary chicken cells, J. Cell. Physiol. 78: 161.

    Article  PubMed  CAS  Google Scholar 

  • Temin, H. M., Pierson, R. W., Jr., and Dulak, N. C., 1972, in: Growth, Nutrition and Metabolism of Cells in Culture, Vol. 1, (H. Rothblat and V. J. Cristafolo, eds.), p. 49, Academic Press, New York, London.

    Google Scholar 

  • Todaro, G. J., Lazar, G. K., and Green, H. J., 1965, The initiation of cell division in a contact-inhibited mammalian cell line, J. Cell. Comp. Physiol. 66: 325.

    Article  CAS  Google Scholar 

  • Uthne, K., 1973, Human somatomedins: Purification and some studies on their biological actions, Acta Endocrinol. Suppl. 175.

    Google Scholar 

  • Wahlström, T., Saksela, E., and Troupp, H., 1973a, Cell-bound antiglial immunity in patients with malignant tumors of the brain, Cell. Immul. 6: 161.

    Article  Google Scholar 

  • Wahlström, T., Linder, E., Saksela, E., and Westermark, B., 1974, Tumor-specific membrane antigens in established cell lines from gliomas, Cancer 34: 274.

    Article  PubMed  Google Scholar 

  • Weiss, R. A., Veselÿ, P., and Sindelarova, J., 1973, Growth regulation and tumor formation of normal and neoplastic rat cells, Int. J. Cancer 11: 77.

    Article  PubMed  CAS  Google Scholar 

  • Westermark, B., 1971, Proliferation control of cultivated human glia-like cells under “steady state” conditions, Exp. Cell Res. 69: 258.

    Article  Google Scholar 

  • Westermark, B., 1973a, Growth control of normal and neoplastic human glia-like cells in culture, Acta Univ. Uppsal. Abst. Uppsala Diss. Med. 164.

    Google Scholar 

  • Westermark, B., 1973b, The deficient density dependent growth control of human malignant glioma cells and virus transformed glia-like cells in culture, Int. J. Cancer 12: 438.

    Article  PubMed  CAS  Google Scholar 

  • Westermark, B., 1973c, Induction of a reversible G1 block in human glia-like cells by cytochalasin B, Exp. Cell Res. 82: 341.

    Article  PubMed  CAS  Google Scholar 

  • Westermark, B., 1973d, Growth regulatory interactions between stationary human glia-like cells and normal neoplastic human cells in culture. I. Normal cells. Exp. Cell Res. 81: 195.

    Article  PubMed  CAS  Google Scholar 

  • Westermark, B., 1973e, Growth regulatory interactions between stationary human glia-like cells and normal and neoplastic human cells in culture. II. Neoplastic cells.

    Google Scholar 

  • Westermark, B., Forsby, N., Brunk, U., Ericsson, J., and Pontén, J., 1972, Studies on in vitro cultivated human glia and glioma cells. I and II, Acta Pathol. Microbiol. Scand. Sect. A 80: 694.

    Google Scholar 

  • Westermark, B., Pontén, J., and Hugosson, R., 1973, Determinants for the establishment of permanent tissue culture lines from human gliomas, Acta Pathol. Microbiol. Scand. Sect. A, Pathol. Microbial. Scand. 81: 791.

    CAS  Google Scholar 

  • Westermark, B., and Wasteson, A., 1974, The response of cultured human normal glial cells to growth factors, Adv. Metab. Dis.,in press.

    Google Scholar 

  • Windle, W. F., 1958, Biology of Neuroglia, Charles C Thomas, Springfield, Ill.

    Google Scholar 

  • Wilson, C. B., Barker, M., and Slagel, D. E., 1966, Tumors of the central nervous system in monolayer tissue, Arch. Neurol. 15: 275.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Cell Biology, The Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden

    Jan Pontén

Authors
  1. Jan Pontén
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Sloan-Kettering Institute for Cancer Research, Donald S. Walker Laboratory, Rye, New York, USA

    Jørgen Fogh

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer Science+Business Media New York

About this chapter

Cite this chapter

Pontén, J. (1975). Neoplastic Human Glia Cells in Culture. In: Fogh, J. (eds) Human Tumor Cells in Vitro . Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1647-4_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-1647-4_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1649-8

  • Online ISBN: 978-1-4757-1647-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation